Sarwath Hina, Bansal Devendra, Husain Nazik Elmalaika, Mohamed Mahmoud, Sultan Ali A, Bedri Shahinaz
Department of Pathology and Laboratory Medicine, Weill Cornell Medicine - Qatar, Cornell University, Qatar Foundation - Education City, Doha, Qatar.
Department of Microbiology and Immunology, Weill Cornell Medicine - Qatar, Cornell University, Qatar Foundation - Education City, Doha, Qatar.
Infect Agent Cancer. 2017 Sep 30;12:50. doi: 10.1186/s13027-017-0159-0. eCollection 2017.
Cervical cancer is the fourth most common cancer in women worldwide with highest incidence reported in Eastern Africa in 2012. The primary goal of this study was to study the expression of p16 in squamous cell carcinoma (SCC) of the cervix by immunohistochemistry (IHC) and determine relation with clinico-pathological parameters. This study further explored the correlation of p16 immunostaining with another proliferation marker, Ki-67 and to study if human papillomavirus (HPV) IHC can be used as a marker for detection of virus in high-grade dysplasia.
A total of 90 samples, diagnosed for cervical cancer, were included in the study. Fixed Paraffin Embedded (FFPE) tissue sections were stained with anti-p16, anti-Ki-67 and anti-HPV antibodies using automated immunohistochemistry platform (ASLink 48-DAKO).
Immunohistochemical protein expression of p16 positivity was found to be highest in SCC (92.2%, = 71) than other HPV tumors (76.9%, = 10). The majority of cases (97.4%) were p16 positive in the age group 41-60 years. In addition, a statistically significant difference between p16 and HPV was observed among total cervical tumor cases and SCC cases.
As expected staining of invasive cervical cancer with anti-HPV showed rare positivity because HPV heralds active infection in dysplastic lesions and not of frank cervical carcinoma. In contrast, anti-p16 IHC results showed positive correlation in SCC and other cervical tumors.
宫颈癌是全球女性中第四大常见癌症，2012年东非报告的发病率最高。本研究的主要目的是通过免疫组织化学（IHC）研究p16在宫颈鳞状细胞癌（SCC）中的表达，并确定其与临床病理参数的关系。本研究进一步探讨了p16免疫染色与另一种增殖标志物Ki-67的相关性，并研究人乳头瘤病毒（HPV）免疫组织化学是否可作为高级别发育异常中病毒检测的标志物。
本研究共纳入90例诊断为宫颈癌的样本。使用自动免疫组织化学平台（ASLink 48 - DAKO）对固定石蜡包埋（FFPE）组织切片进行抗p16、抗Ki-67和抗HPV抗体染色。
发现p16阳性的免疫组织化学蛋白表达在SCC中最高（92.2%，n = 71），高于其他HPV肿瘤（76.9%，n = 10）。在41 - 60岁年龄组中，大多数病例（97.4%）p16呈阳性。此外，在所有宫颈肿瘤病例和SCC病例中，观察到p16和HPV之间存在统计学显著差异。
正如预期的那样，抗HPV对浸润性宫颈癌的染色显示罕见的阳性，因为HPV预示着发育异常病变中的活跃感染，而非明显的宫颈癌。相比之下，抗p16免疫组织化学结果在SCC和其他宫颈肿瘤中显示出正相关。
