Molecular Imaging & Therapy Branch, National Cancer Center, 111 Jungbalsan-ro, Ilsandong-gu, Goyang, Gyeonggi-do 410-769, South Korea.
ACS Nano. 2011 Feb 22;5(2):1086-94. doi: 10.1021/nn102722z. Epub 2011 Jan 18.
A gold nanorod (GNR)-photosensitizer complex was developed for noninvasive near-infrared fluorescence imaging and cancer therapy. We showed that (a) fluorescence emission and singlet oxygen generation by AlPcS(4) were quenched after complex formation with GNRs; (b) 4-fold greater intracellular uptake and better in vitro phototoxicity were observed in GNR-AlPcS(4)-treated cells than in free AlPcS(4)-treated cells; and (c) after intravenous injection of the GNR-AlPcS(4) complex, tumor sites were clearly identified on near-infrared fluorescence images as early as 1 h after injection. The tumor-to-background ratio increased over time and was 3.7 at 24 h; tumor growth reduced by 79% with photodynamic therapy (PDT) alone and by 95% with dual photothermal therapy (PTT) and PDT. This novel multifunctional nanomedicine may be useful for near-infrared fluorescence imaging and PTT/PDT in various cancers.
一种金纳米棒（GNR）-光敏剂复合物被开发用于非侵入性近红外荧光成像和癌症治疗。我们表明：（a）AlPcS（4）的荧光发射和单线态氧生成在与 GNR 形成复合物后被猝灭；（b）与游离 AlPcS（4）处理的细胞相比，GNR-AlPcS（4）处理的细胞中观察到细胞内摄取增加了 4 倍，体外光毒性更好；（c）静脉注射 GNR-AlPcS（4）复合物后，早在注射后 1 小时，即可在近红外荧光图像上清晰识别肿瘤部位。肿瘤与背景的比值随时间增加，24 小时时达到 3.7；单独光动力疗法（PDT）可使肿瘤生长减少 79%，光热疗法（PTT）和 PDT 联合可使肿瘤生长减少 95%。这种新型多功能纳米医学可能对各种癌症的近红外荧光成像和 PTT/PDT 有用。
