Department of Animal Nutrition, Faculty of Veterinary Science, Firat University, Elazig, Turkey.
J Anim Physiol Anim Nutr (Berl). 2012 Feb;96(1):66-74. doi: 10.1111/j.1439-0396.2010.01123.x. Epub 2011 Jan 19.
In the present study, the effects of dietary resveratrol on the induction of heat shock proteins, transcription factors and antioxidative enzyme system in liver of quails under heat stress were investigated. A total of 180 (55-day-old) female Japanese quails (Coturnix coturnix japonica) were reared either at 22 °C for 24 h/day (thermoneutral, TN) or 34 °C for 8 h/day (heat stress, HS; 09:00-17:00 hours) for 12 weeks. Birds in both environments were randomly fed one of three diets: basal diet and basal diet added with either 200 or 400 mg of resveratrol per kg of diet. The results showed that exposure to high ambient temperature induced decreases in feed intake, egg production, and hepatic superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities but increases in hepatic malondialdehyde (MDA) concentrations (p < 0.001). Liver Hsp70, Hsp90 and NF-κB expression was greater while Nrf2 expression was lower for quails reared under the heat stress than for those reared under the TN environment (p < 0.0001). There were linear increases in feed intake, egg production, hepatic SOD, CAT, and GSH-Px activities as well as Nrf2 expression, but linear decreases in hepatic MDA concentrations and Hsp70, Hsp90, and NF-κB expressions with increasing supplemental resveratrol level (p < 0.0001). Two-way treatment interactions revealed that the degree of restorations in all response variables was more notable under the high ambient temperature than that of the TN environment as dietary resveratrol concentration was increased. The results of the present study suggest that supplemental resveratrol reduces oxidative stress in heat-stressed quails through modulating the hepatic heat shock proteins and nuclear transcription factors.
本研究探讨了饲粮白藜芦醇对热应激鹌鹑肝脏热休克蛋白、转录因子和抗氧化酶系统诱导的影响。将 180 只(55 日龄)雌性日本鹌鹑(Coturnix coturnix japonica)分别在 22°C(常温,TN)或 34°C(热应激,HS;09:00-17:00 小时)下饲养 12 周,每天 24 小时。两组环境中的鸟类随机饲喂三种日粮之一:基础日粮和基础日粮分别添加 200 或 400 mg/kg 白藜芦醇。结果表明,暴露于高温环境会降低采食量、产蛋量和肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性,但增加肝脏丙二醛(MDA)浓度(p<0.001)。与在 TN 环境下饲养的鹌鹑相比,在 HS 环境下饲养的鹌鹑肝脏 Hsp70、Hsp90 和 NF-κB 表达增加,而 Nrf2 表达降低(p<0.0001)。随着饲粮白藜芦醇水平的增加,采食量、产蛋量、肝脏 SOD、CAT 和 GSH-Px 活性以及 Nrf2 表达呈线性增加,而肝脏 MDA 浓度和 Hsp70、Hsp90 和 NF-κB 表达呈线性降低(p<0.0001)。双向处理相互作用表明,随着饲粮白藜芦醇浓度的增加,高温环境下所有应答变量的恢复程度比 TN 环境下更为显著。本研究表明,饲粮添加白藜芦醇通过调节肝脏热休克蛋白和核转录因子来减轻热应激鹌鹑的氧化应激。