Hekker A C, Brand-Saathof B, Vis J, Meijers R C
J Infect Dis. 1979 Oct;140(4):596-600. doi: 10.1093/infdis/140.4.596.
A technique for the detection of IgM antibodies to cytomegalovirus (CMV) by immunofluorescence was developed. In order to prevent interference by rheumatoid factor in the sera, IgG was removed by prior immunoabsorption with antiserum to gamma Fc. Sera from 63 patients with a rise in titer of antibody to CMV, indicated by complement fixation, were IgM-positive, but yielded negative results on the Paul-Bunnell-Davidsohn test for infectious mononucleosis. Convalescent-phase serum samples from 20 patients with a seroconversion to herpes simplex virus and from 20 patients with a seroconversion to varicella-zoster virus also had no IgM antibodies to CMV. Sera from six of 10 patients with infectious mononucleosis and two of 100 normal blood donors were positive for IgM antibodies to CMV. In 38 of 63 patients, the diagnosis of CMV infection could be made several weeks earlier by the immunofluorescence test than by the complement-fixation test. IgM antibodies to CMV persisted for more than two months after onset of symptoms of the infection.
开发了一种通过免疫荧光检测巨细胞病毒（CMV）IgM抗体的技术。为防止血清中类风湿因子的干扰，先用抗γFc抗血清进行免疫吸附去除IgG。63例通过补体结合试验显示CMV抗体滴度升高的患者血清，IgM呈阳性，但在传染性单核细胞增多症的保罗-邦内尔-戴维森试验中结果为阴性。20例单纯疱疹病毒血清转化患者和20例水痘-带状疱疹病毒血清转化患者的恢复期血清样本也没有CMV IgM抗体。10例传染性单核细胞增多症患者中有6例血清以及100例正常献血者中有2例血清CMV IgM抗体呈阳性。63例患者中有38例，通过免疫荧光试验诊断CMV感染可比补体结合试验提前数周。CMV IgM抗体在感染症状出现后持续两个多月。
