Buimovici-Klein E, Tinker M K, O'Beirne A J, Lange M, Cooper Z
Arch Virol. 1983;78(3-4):203-12. doi: 10.1007/BF01311315.
A new commercially developed cytomegalovirus (CMV)-IgM ELISA was found to be sensitive and specific when compared with sucrose gradient fractionation of Ig classes in CMV antibody-positive and negative sera. The presence of CMV IgM in patients' sera correlated with positive virus isolation from circulating mononuclear blood cells and urine. Serial examinations of patients with primary or recent CMV infection revealed a typical sequence of IgM and IgG development. The frequency of CMV isolation declined as the concentration of IgM decreased and the IgG levels increased. Since the isolation of CMV from clinical specimens is a cumbersome procedure, we suggest that the IgM ELISA could provide rapid and valuable information on the presence of an active or reactivated CMV infection.
与巨细胞病毒（CMV）抗体阳性和阴性血清中免疫球蛋白（Ig）类别的蔗糖梯度分级分离法相比，一种新的商业开发的CMV-IgM酶联免疫吸附测定法被发现具有敏感性和特异性。患者血清中CMV IgM的存在与从循环单核血细胞和尿液中分离出阳性病毒相关。对原发性或近期CMV感染患者的系列检查揭示了IgM和IgG产生的典型序列。随着IgM浓度降低和IgG水平升高，CMV分离的频率下降。由于从临床标本中分离CMV是一个繁琐的过程，我们建议IgM酶联免疫吸附测定法可为活动性或再激活的CMV感染的存在提供快速且有价值的信息。
