Lonchampt M O, Goulin J, Pinelis V, Chabrier P E, Braquet P
Institut Henri Beaufour, Les Ulis, France.
Arch Mal Coeur Vaiss. 1990 Jul;83(8):1309-11.
In order to examine the effect of endothelin (ET-1) as a growth factor, vascular smooth muscle cells were isolated from aortas of spontaneously hypertensive (SHR) and control (WKY) rats. Cell proliferation was determined by measurement of labelled 3H-thymidine incorporation in quiescent cells in presence of ET-1 alone or in association with another growth factor, insulin (INS). ET-1 alone (0.1 to 100 nM) increased slightly the growth of both types of cells. This effect was enhanced in presence of INS (0.1 to 10 micrograms/ml). SHR cells were more reactive than control ones. Activation of Na+/H+ exchange which is a necessary response for mitogenesis was dose dependently observed with increasing concentrations of ET-1 (0.1 to 100 nM) further confirming that ET-1 could act as a growth factor for vascular smooth muscle cells.
为了研究内皮素（ET-1）作为一种生长因子的作用，从自发性高血压大鼠（SHR）和对照大鼠（WKY）的主动脉中分离出血管平滑肌细胞。通过测量单独存在ET-1或与另一种生长因子胰岛素（INS）联合存在时，静止细胞中标记的3H-胸腺嘧啶核苷掺入量来确定细胞增殖。单独的ET-1（0.1至100 nM）可轻微增加两种类型细胞的生长。在INS（0.1至10微克/毫升）存在下，这种作用增强。SHR细胞比对照细胞更具反应性。随着ET-1浓度增加（0.1至100 nM），剂量依赖性地观察到Na+/H+交换的激活，这是有丝分裂的必要反应，进一步证实ET-1可作为血管平滑肌细胞的生长因子。
