山羊α-乳白蛋白作为活性蛋白在酿酒酵母中的表达与分泌。

Expression and secretion of goat alpha-lactalbumin as an active protein in Saccharomyces cerevisiae.

作者信息

Takeda S, Tamaki E, Miura K, Kumagai I

机构信息

Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Dec 14;173(2):741-7. doi: 10.1016/s0006-291x(05)80098-1.

DOI:10.1016/s0006-291x(05)80098-1

PMID:2124489

Abstract

An expression plasmid for goat alpha-lactalbumin in Saccharomyces cerevisiae, pSKA100, was constructed into a shuttle vector, pYG100, by inserting cDNA which encodes goat pre-alpha-lactalbumin and two-thirds of the 3'-non-coding region. The goat alpha-lactalbumin was expressed under the yeast glyceraldehyde 3-phosphate dehydrogenase (GPD) promoter and terminator of pYG100 and secreted in the growth medium for yeast as a precise mature protein, possessing specific activity essentially the same as that of authentic goat alpha-lactalbumin in lactose synthesis.

摘要

通过插入编码山羊前α-乳白蛋白和三分之二3'-非编码区的cDNA，将用于在酿酒酵母中表达山羊α-乳白蛋白的表达质粒pSKA100构建到穿梭载体pYG100中。山羊α-乳白蛋白在pYG100的酵母甘油醛-3-磷酸脱氢酶（GPD）启动子和终止子的控制下表达，并作为一种精确的成熟蛋白分泌到酵母生长培养基中，其比活性与乳糖合成中天然山羊α-乳白蛋白的比活性基本相同。