Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA.
Nucleic Acids Res. 2011 May;39(9):3962-71. doi: 10.1093/nar/gkq1288. Epub 2011 Jan 17.
We report here the first synthesis of 5-phenyl-telluride-thymidine derivatives and the Te-phosphoramidite. We also report here the synthesis, structure and STM current-imaging studies of DNA oligonucleotides containing the nucleobases (thymine) derivatized with 5-phenyl-telluride functionality (5-Te). Our results show that the 5-Te-DNA is stable, and that the Te-DNA duplex has the thermo-stability similar to the corresponding native duplex. The crystal structure indicates that the 5-Te-DNA duplex structure is virtually identical to the native one, and that the Te-modified T and native A interact similarly to the native T and A pair. Furthermore, while the corresponding native showed weak signals, the DNA duplex modified with electron-rich tellurium functionality showed strong topographic and current peaks by STM imaging, suggesting a potential strategy to directly image DNA without structural perturbation.
我们首次合成了 5-苯基碲代胸苷衍生物和碲亚磷酸酯。我们还报告了含有（胸腺嘧啶）衍生 5-苯基碲功能化的寡核苷酸的合成、结构和 STM 电流成像研究。我们的结果表明，5-Te-DNA 是稳定的，并且 Te-DNA 双链体具有与相应天然双链体相似的热稳定性。晶体结构表明，5-Te-DNA 双链体结构几乎与天然双链体相同，并且 Te 修饰的 T 和天然的 A 与天然的 T 和 A 对相互作用相似。此外，虽然相应的天然物显示出较弱的信号，但用富电子碲官能团修饰的 DNA 双链体通过 STM 成像显示出强烈的形貌和电流峰，这表明了一种无需结构干扰即可直接成像 DNA 的潜在策略。
