Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1903-7. doi: 10.1073/pnas.1019267108. Epub 2011 Jan 18.
Tissue differentiation is an important process that involves major cellular membrane remodeling. We used Madin-Darby canine kidney cells as a model for epithelium formation and investigated the remodeling of the total cell membrane lipidome during the transition from a nonpolarized morphology to an epithelial morphology and vice versa. To achieve this, we developed a shotgun-based lipidomics workflow that enabled the absolute quantification of mammalian membrane lipidomes with minimal sample processing from low sample amounts. Epithelial morphogenesis was accompanied by a major shift from sphingomyelin to glycosphingolipid, together with an increase in plasmalogen, phosphatidylethanolamine, and cholesterol content, whereas the opposite changes took place during an epithelial-to-mesenchymal transition. Moreover, during polarization, the sphingolipids became longer, more saturated, and more hydroxylated as required to generate an apical membrane domain that serves as a protective barrier for the epithelial sheet.
组织分化是一个重要的过程，涉及主要的细胞内膜重塑。我们使用 Madin-Darby 犬肾细胞作为上皮形成的模型，研究了从非极化形态到上皮形态的转变过程中以及反之亦然时整个细胞膜脂类组的重塑。为此，我们开发了一种基于 shotgun 的脂质组学工作流程，该流程可实现从少量样品中进行最小样品处理，对哺乳动物膜脂类组进行绝对定量。上皮形态发生伴随着鞘磷脂向糖脂的重大转变，同时鞘磷脂、神经酰胺、心磷脂、磷脂酰乙醇胺和胆固醇含量增加，而在上皮细胞向间充质转化过程中则发生相反的变化。此外，在极化过程中，鞘脂的长度增加、饱和度增加且羟基化增加，以产生作为上皮片保护屏障的顶端膜结构域。
