Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695-8002, USA.
Heredity (Edinb). 2011 Aug;107(2):105-14. doi: 10.1038/hdy.2010.168. Epub 2011 Jan 19.
Loblolly pine, Pinus taeda L., is one of the most widely planted, commercially and ecologically important tree species in North America. We took an association genetics approach, using an unimproved population of 380 clonally replicated unrelated trees, to test 3,938 single nucleotide polymorphisms (SNPs) in as many genes for association with phenotypic variation in carbon isotope discrimination, foliar nitrogen concentration and total tree height after two growing seasons. Best linear unbiased prediction (BLUP) was used with a spatial adjustment to remove environmental variation from phenotypic data derived from a common garden experiment. After correction for multiple testing, a total of 14 SNPs were associated with the traits of carbon isotope discrimination (n = 7), height (n = 1) and foliar nitrogen concentration (n = 6) using 380 clones. Tails of the population phenotypic distribution were compared for allele frequency differences, revealing 10 SNPs with allele frequency in at least one tail significantly different from the overall population. Eight associated SNPs were in sequences similar to known genes, such as an AP2 transcription factor related to carbon isotope discrimination and glutamate decarboxylase associated with foliar nitrogen concentration, and others were from unknown genes without homologs in Arabidopsis.
火炬松（ Pinus taeda L.）是北美地区种植最广泛、商业和生态上最重要的树种之一。我们采用关联遗传学方法，利用未经改良的 380 棵无性系复制的无关树木的群体，对 3938 个单核苷酸多态性（SNP）进行关联分析，以检验其与碳同位素分馏、叶片氮浓度和两个生长季后的总树高的表型变异之间的关系。最佳线性无偏预测（BLUP）与空间调整相结合，从共同花园实验中获得的表型数据中去除环境变异。经过多重检验校正后，共有 14 个 SNP 与碳同位素分馏（n=7）、高度（n=1）和叶片氮浓度（n=6）的性状相关，使用了 380 个克隆。对群体表型分布的尾部进行等位基因频率差异比较，发现 10 个 SNP 的等位基因频率在至少一个尾部与总体群体显著不同。8 个相关 SNP 位于与已知基因相似的序列中，例如与碳同位素分馏相关的 AP2 转录因子和与叶片氮浓度相关的谷氨酸脱羧酶，而其他 SNP 则来自于没有拟南芥同源物的未知基因。
