Kacerovský M, Drahosová M, Hornychová H, Plísková L, Bolehovská R, Förstl M, Tosner J, Lesko D, Andrýs C
Lékarská fakulta Hradec Králové, Univerzita Karlova Praha, Porodnická a gynekologická klinika FN Hradec Králové.
Ceska Gynekol. 2009 Dec;74(6):403-10.
The purpose of this study was to determinate the changes of amniotic fluid interleukin 6 (IL-6) concentrations in patients with preterm premature rupture of the membranes (PPROM), and in the presence of microbial invasion of the amniotic cavity (MIAC) and histological chorioamnionitis (HCA). The aim was to examine amniotic fluid IL-6 in relation to MIAC and HCA.
Prospective study.
Department of Obstetrics and Gynecology Medical Faculty Charles University Hradec Králové.
We studied 37 women between 24 and 36 weeks of gestation with PPROM. Samples of amniotic fluid were collected by transabdominal amniocentesis. Polymerase chain reaction for the genital mycoplasmas and culture for aerobic and anaerobic bacteria were performed. Twenty-eight of 37 patients placentas were collected and assessed for presence or absence HCA. IL-6 concentration in amniotic fluid were determined using a sensitive and specific diagnostic kit Human IL-6 Quantikine ELISA manufactured R&D Systems, USA.
There was significant difference in the median amniotic fluid IL-6 concentration between patients with preterm rupture of the membranes with and without MIAC and HCA (patients with MIAC and HCA: median 915 pg/ml, range 651-1854 pg/ml vs. patients without MIAC and HCA: median 780 pg/ml, range 184-1059 pg/ml; p=0.047). There was no significant difference in the median amniotic fluid IL-6 concentration between patients with preterm rupture of the membranes with and without MIAC (patients with MIAC: median 915 pg/ml, range 195-1854 pg/ml vs. patients without MIAC: median 792 pg/ml, range 184-1993 pg/ml; p=0.53). There was no significant difference in the median amniotic fluid IL-6 concentration between patients with preterm rupture of the membranes with and without HCA (patients with HCA: median 829 pg/ml, range 195-1992 pg/ml vs. patients without HCA: median 768 pg/ml, range 184-1890 pg/ml; p = 0.31).
Amniotic fluid IL-6 concentrations patients with PPROM with presence HCA and MIAC were significantly higher than IL-6 concentration patients without HCA and MIAC.
本研究旨在确定胎膜早破（PPROM）患者、存在羊膜腔微生物入侵（MIAC）和组织学绒毛膜羊膜炎（HCA）时羊水白细胞介素6（IL-6）浓度的变化。目的是研究羊水IL-6与MIAC和HCA的关系。
前瞻性研究。
布拉格查理大学医学院妇产科。
我们研究了37名孕周在24至36周之间的PPROM患者。通过经腹羊膜腔穿刺收集羊水样本。进行了生殖支原体的聚合酶链反应以及需氧菌和厌氧菌培养。收集了37例患者中的28例胎盘，评估是否存在HCA。使用美国R&D Systems公司生产的灵敏且特异的诊断试剂盒人IL-6 Quantikine ELISA测定羊水中IL-6的浓度。
有MIAC和HCA的胎膜早破患者与无MIAC和HCA的胎膜早破患者羊水IL-6浓度中位数存在显著差异（有MIAC和HCA的患者：中位数915 pg/ml，范围651 - 1854 pg/ml；无MIAC和HCA的患者：中位数780 pg/ml，范围184 - 1059 pg/ml；p = 0.047）。有MIAC的胎膜早破患者与无MIAC的胎膜早破患者羊水IL-6浓度中位数无显著差异（有MIAC的患者：中位数915 pg/ml，范围195 - 1854 pg/ml；无MIAC的患者：中位数792 pg/ml，范围184 - 1993 pg/ml；p = 0.53）。有HCA的胎膜早破患者与无HCA的胎膜早破患者羊水IL-6浓度中位数无显著差异（有HCA的患者：中位数829 pg/ml，范围195 - 1992 pg/ml；无HCA的患者：中位数768 pg/ml，范围184 - 1890 pg/ml；p = 0.31）。
存在HCA和MIAC的PPROM患者羊水IL-6浓度显著高于无HCA和MIAC的患者羊水IL-6浓度。
