Yao Zongling, Du Shouying, Lu Yang, Xu Bing, Chen Xiaolan
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Zhongguo Zhong Yao Za Zhi. 2010 Oct;35(20):2696-9.
To develop a RP-HPLC method for the simultaneous determination of five components including ligustrazine, ferulic acid, butylphthalide, ligustilide and butene-NBP in Rhizoma Chuanxiong, a Chinese herbal medicine.
The chromatographic analysis was carried out by using a grace smart RP C18 column with the mobile phase consisted of acetonitrile and 0.1% phosphoric acid (volume fraction), which was in gradient elution. A DAD detector was used to detect the components. The peak area was chosen under their maximum absorption wavelength for different components, and quantitated by using the external standard method.
The calibration curves were linear within the range of 0.008 35-0.668, 0.020 6-1.648, 0.012 2-0.976 , 0.050 75-4.06, 0.015 7-1.256 microg for ligustrazine, ferulic acid, butylphthalide, ligustilide and butene-NBP, respectively. The average recoveries of the five components were 101.98%, 99.91%, 96.94%, 100.85% and 99.04%, respectively.
This method is simple, quick, reproducible, with high recovery, and has been successfully applied to the simultaneous determination of the five components in Rhizoma Chuanxiong. This method can be used to control the quality of Rhizoma Chuanxiong and its preparations.
建立一种反相高效液相色谱法（RP-HPLC），用于同时测定中药川芎中包括川芎嗪、阿魏酸、丁苯酞、藁本内酯和丁烯基苯酞在内的五种成分。
采用Grace Smart RP C18色谱柱进行色谱分析，流动相为乙腈和0.1%磷酸（体积分数），进行梯度洗脱。使用二极管阵列检测器（DAD）检测各成分。在不同成分的最大吸收波长下选择峰面积，采用外标法进行定量。
川芎嗪、阿魏酸、丁苯酞、藁本内酯和丁烯基苯酞的校准曲线在0.008 35 - 0.668、0.020 6 - 1.648、0.012 2 - 0.976、0.050 75 - 4.06、0.015 7 - 1.256 μg范围内呈线性。五种成分的平均回收率分别为101.98%、99.91%、96.94%、100.85%和99.04%。
该方法简便、快速、重现性好、回收率高，已成功应用于川芎中五种成分的同时测定。该方法可用于控制川芎及其制剂的质量。
