Laboratory of GlycoNanotechnology, Biofunctional Nanomaterials Unit, CICbiomaGUNE and CIBER-BBN, P° de Miramón 182, 20009 San Sebastian, Spain.
Bioconjug Chem. 2011 Feb 16;22(2):264-73. doi: 10.1021/bc1003923. Epub 2011 Jan 19.
A versatile nanoplatform based on magnetic glyconanoparticles (glyco-ferrites) to attach well-oriented antibodies is described. An efficient ligand exchange process has been used to prepare water-soluble 6-nm-sized core-shell Fe(3)O(4)@Au nanoparticles bearing amphiphilic carbohydrates and aliphatic ethylene glycol chains ended in a carboxyl group. The covalent immobilization through the carboxyl group of an Fc receptor (protein G) enables successful well-oriented capture of immunoglobulins G onto the magnetic glyconanoparticle. A thorough characterization of structure and biofunctionality of the constructs is carried out by different techniques. The selective immunolabeling of cells by the antibody-magnetic glyconanoparticle conjugates is demonstrated by magnetic resonance imaging (MRI), as well as by fluorescence techniques.
一种基于磁性糖纳米粒子（糖铁氧体）的多功能纳米平台，用于附着定向良好的抗体。已使用高效配体交换过程制备水溶性 6nm 大小的核壳 Fe3O4@Au 纳米粒子，其具有两亲性碳水化合物和末端带有羧基的脂肪族乙二醇链。通过羧基的共价固定化使得 Fc 受体（蛋白 G）能够成功地将免疫球蛋白 G 定向捕获到磁性糖纳米粒子上。通过不同的技术对结构和生物功能进行了全面的表征。通过磁共振成像（MRI）以及荧光技术证明了抗体-磁性糖纳米粒子缀合物对细胞的选择性免疫标记。
