Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, PR China.
Int J Mol Med. 2011 Mar;27(3):429-34. doi: 10.3892/ijmm.2011.603. Epub 2011 Jan 18.
Cysteine-rich 61 (Cyr61), a secreted protein which belongs to the CCN family, has been found to be differentially expressed in many cancers and to be involved in tumor progression. The expression of Cyr61 in esophageal squamous cell carcinoma (ESCC) has only recently been described, but the roles of Cyr61 in ESCC cells still remained unclear. In this study, we have shown that there are high levels of Cyr61 in ESCC cell lines. Furthermore, using RNA interference (RNAi), we stably silenced the expression of Cyr61 in EC109 cells, an ESCC cell line. The colony formation, MTT, cell migration, cell invasiveness and cell adhesion assays were employed to address the roles of Cyr61 in the growth, migration and adhesion of ESCC cells. The results have shown that Cyr61 knockdown by RNAi leads to a significant reduction of colony formation and cell growth. The migration and invasiveness ability of EC109 cells were also suppressed with the Cyr61 down-regulation. Furthermore, the adhesion of the EC109 cells was decreased in the Cyr61 knockdown cells compared to the control cells. Taken together, our data suggest that Cyr61 may play crucial roles in regulating neoplasm progression of ESCC.
富含半胱氨酸的 61 型（Cyr61）是一种分泌蛋白，属于 CCN 家族，已被发现在许多癌症中差异表达，并参与肿瘤进展。Cyr61 在食管鳞状细胞癌（ESCC）中的表达最近才被描述，但 Cyr61 在 ESCC 细胞中的作用仍不清楚。在这项研究中，我们表明 ESCC 细胞系中存在高水平的 Cyr61。此外，我们通过 RNA 干扰（RNAi）稳定沉默 ESCC 细胞系 EC109 中 Cyr61 的表达。通过集落形成、MTT、细胞迁移、细胞侵袭和细胞黏附测定来研究 Cyr61 在 ESCC 细胞生长、迁移和黏附中的作用。结果表明，RNAi 下调 Cyr61 导致集落形成和细胞生长显著减少。下调 Cyr61 还抑制了 EC109 细胞的迁移和侵袭能力。此外，与对照细胞相比，Cyr61 下调细胞中 EC109 细胞的黏附能力降低。总之，我们的数据表明 Cyr61 可能在调节 ESCC 的肿瘤进展中发挥关键作用。
