Huang Jianhua, Gao Kanda, Lin Jian, Wang Qiugen
Department of Orthopaedics, Affiliated People's First Hospital, Shanghai Jiaotong University, 200080, Shanghai, China.
Tumour Biol. 2014 Feb;35(2):1095-100. doi: 10.1007/s13277-013-1146-8. Epub 2013 Dec 10.
Increasing evidence indicates that microRNAs (miRNAs) participate in almost every step of cellular processes and are often aberrantly expressed in human cancer. Therefore, the discovery of miRNAs may provide a new and powerful tool for understanding the mechanism and treatment of carcinogenesis. The aim of this study was to investigate the functional significance of miR-100 and to identify its possible target genes in osteosarcoma (OS) cells. Here, we found that expression level of miR-100 was significantly decreased in osteosarcoma tissues in comparison with the adjacent normal tissues. The enforced expression of miR-100 was able to inhibit cell proliferation in Saos-2 and MG63 cells, while its antisense oligonucleotides (antisense miR-100) promoted cell proliferation. Moreover, our results further revealed that expression of Cyr61, an extracellular matrix-associated growth factor, was negatively regulated by miR-100. Therefore, we consider that miR-100 acts as a tumor suppressor for osteosarcoma. It may provide novel diagnostic and therapeutic options for human osteosarcoma in the future.
越来越多的证据表明，微小RNA（miRNA）参与细胞过程的几乎每个步骤，并且在人类癌症中经常异常表达。因此，miRNA的发现可能为理解致癌机制和治疗提供一种新的有力工具。本研究的目的是探讨miR-100的功能意义，并在骨肉瘤（OS）细胞中鉴定其可能的靶基因。在这里，我们发现与相邻正常组织相比，骨肉瘤组织中miR-100的表达水平显著降低。miR-100的强制表达能够抑制Saos-2和MG63细胞的增殖，而其反义寡核苷酸（反义miR-100）则促进细胞增殖。此外，我们的结果进一步揭示，细胞外基质相关生长因子Cyr61的表达受到miR-100的负调控。因此，我们认为miR-100作为骨肉瘤的肿瘤抑制因子。它可能在未来为人类骨肉瘤提供新的诊断和治疗选择。
