Ozzello L, Habif D V, DeRosa C M
Arthur Purdy Stout Laboratory of Surgical Pathology, Columbia University, New York, New York.
Breast Cancer Res Treat. 1990 Sep;16(2):89-96. doi: 10.1007/BF01809292.
Nude mice bearing bilateral xenografts of human breast carcinoma cells (MCF-7 and BT20) were treated with 2 or 45-day cycles of intralesional (i.l.) injections of human natural interferon beta (nIFN-beta) alone or in combination with human natural interferon gamma (nIFN-gamma). The injections were administered to only 1 of the 2 tumors in each animal, thus making it possible to assess at the same time local therapeutic effects in the injected tumors and systemic effects in the contralateral ones. When n-IFN-beta was used as a single agent only mild local antitumor effects and virtually no systemic effects were observed. In contrast, the combined administration of nIFN-beta/nIFN-gamma produced marked antiproliferative effects, presumably as a result of the synergistic action of type I and type II IFNs. These effects ranged from complete regression documented histologically in 2 MCF-7 tumors to varying degrees of growth inhibition with persistence of residual microscopic or grossly detectable tumor. Local effects were more pronounced than systemic effects. The therapeutic efficacy of nIFN-beta proved to be greater than that of recombinant interferon beta (rIFN-beta). In MCF-7 tumors nIFN-beta appeared to be less effective than nIFN-alpha, whereas the opposite was true for BT20 tumors.
用人乳腺癌细胞（MCF - 7和BT20）双侧异种移植的裸鼠，接受了单独或与人天然干扰素γ（nIFN - γ）联合的人天然干扰素β（nIFN - β）瘤内（i.l.）注射，注射周期为2天或45天。每次注射仅针对每只动物的2个肿瘤中的1个，这样就可以同时评估注射肿瘤的局部治疗效果和对侧肿瘤的全身效果。当单独使用n - IFN - β时，仅观察到轻微的局部抗肿瘤作用，几乎没有全身作用。相反，nIFN - β/nIFN - γ联合给药产生了显著的抗增殖作用，这可能是由于I型和II型干扰素的协同作用。这些作用范围从2个MCF - 7肿瘤组织学记录的完全消退到不同程度的生长抑制，残留微小或可肉眼检测到的肿瘤持续存在。局部作用比全身作用更明显。nIFN - β的治疗效果被证明大于重组干扰素β（rIFN - β）。在MCF - 7肿瘤中，nIFN - β似乎比nIFN - α效果差，而在BT20肿瘤中则相反。
