Ida N, Uenishi N, Kajita A, Satoh Y
Gan. 1982 Dec;73(6):952-60.
The antitumor effect of human fibroblast interferon (HuIFN-beta) on the growth of human melanoma cells (HMV-1) in vitro and in vivo was examined. In in vitro experiments, HMV-1 cells were highly sensitive to HuIFN-beta compared to other human cells. The cell growth in nude mice was also suppressed by daily administration of HuIFN-beta, depending on the dosage and administration route. The most prominent effect was obtained by intratumoral injection and a lesser therapeutic effect was achieved by subcutaneous injection around the inoculated tumor. Intraperitoneal injection of HuIFN-beta was unsuccessful. Pharmacokinetic studies of HuIFN-beta in nude mouse indicated that the three administration routes gave similar blood plasma levels and decline curves, suggesting that there is no correlation between the plasma HuIFN-beta level and the therapeutic efficacy. However, a relatively high interferon titer was detected for a long time after intratumoral injection of HuIFN-beta. Because of the species specificity of interferon and the use of the nude mouse system lacking T-cell function, the antitumor effect observed in the present study might reflect an anticellular activity of HuIFN-beta retained in the tumor region.
研究了人成纤维细胞干扰素（HuIFN-β）对人黑色素瘤细胞（HMV-1）体外及体内生长的抗肿瘤作用。在体外实验中，与其他人类细胞相比，HMV-1细胞对HuIFN-β高度敏感。裸鼠体内的细胞生长也受到每日给予HuIFN-β的抑制，这取决于剂量和给药途径。瘤内注射获得的效果最显著，接种肿瘤周围皮下注射的治疗效果较差。腹腔注射HuIFN-β未成功。对裸鼠体内HuIFN-β的药代动力学研究表明，三种给药途径的血浆水平和下降曲线相似，这表明血浆HuIFN-β水平与治疗效果之间没有相关性。然而，瘤内注射HuIFN-β后很长一段时间都检测到相对较高的干扰素滴度。由于干扰素的种属特异性以及使用缺乏T细胞功能的裸鼠系统，本研究中观察到的抗肿瘤作用可能反映了HuIFN-β在肿瘤区域保留的抗细胞活性。
