Dawkins H J, Johnson R B, Spencer T L, Patten B E
Department of Agriculture and Rural Affairs, Regional Veterinary Laboratory, Victoria, Australia.
Res Vet Sci. 1990 Nov;49(3):261-7.
Haemorrhagic septicaemia (HS) is caused by specific serotypes of Pasteurella multocida and is one of the major economic diseases of cattle and buffalo in South East Asia. Definitive diagnosis of the disease-causing organism with the available methods is labour intensive and not totally reliable, consequently, an ELISA system to identify P multocida organisms which cause HS was developed. One hundred and twenty-four P multocida isolates were tested, 58 were type strains and 66 were field isolates. Analysis of these strains indicated the assay had a specificity of 99 per cent and sensitivity of at least 86 per cent. The sensitivity could be an underestimate, as five isolates assumed to be false negative reactions may not all be HS-causing strains. The HS ELISA provides a rapid, simple, accurate and inexpensive diagnostic assay for identification of HS causing organisms but does not represent a new typing system for P multocida. This assay will also enable countries to assess the impact of HS more accurately.
出血性败血症（HS）由多杀性巴氏杆菌的特定血清型引起，是东南亚牛和水牛的主要经济疾病之一。用现有方法对致病微生物进行确诊既费力又不完全可靠，因此，开发了一种用于鉴定引起HS的多杀性巴氏杆菌的ELISA系统。对124株多杀性巴氏杆菌分离株进行了检测，其中58株为标准菌株，66株为野外分离株。对这些菌株的分析表明，该检测方法的特异性为99%，敏感性至少为86%。由于5株被认为是假阴性反应的分离株可能并非都是引起HS的菌株，因此敏感性可能被低估。HS ELISA为鉴定引起HS的微生物提供了一种快速、简单、准确且廉价的诊断方法，但并不代表一种新的多杀性巴氏杆菌分型系统。该检测方法还将使各国能够更准确地评估HS的影响。
