利用多位点序列分型和脉冲场凝胶电泳技术对来自巴基斯坦和泰国的出血性败血症相关多杀巴斯德氏菌分离株进行分子分型。
Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis.
机构信息
School of Veterinary and Life Sciences, Murdoch University, South Street, Murdoch, Western Australia 6150, Australia.
出版信息
Res Vet Sci. 2013 Dec;95(3):986-90. doi: 10.1016/j.rvsc.2013.07.003. Epub 2013 Aug 3.
A comparative genetic study of 23 field isolates and vaccine strains of Pasteurella multocida associated with haemorrhagic septicaemia cases from Pakistan and Thailand was done using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The MLST sequence type (ST) for all 20 of the 23 isolates tested was 122. The PFGE results showed one band difference between the Pakistani and the Thai isolates. Sequence type 122 is the dominant associated profile with haemorrhagic septicaemia (HS) cases in South Asia. The study supports the concept of using PFGE for short-term epidemiology and MLST for long-term epidemiology.
对来自巴基斯坦和泰国的与出血性败血症病例相关的 23 株巴氏杆菌田间分离株和疫苗株进行了比较遗传研究，方法是使用脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）。对测试的 23 株分离株中的 20 株进行了 MLST 序列型（ST）分析，结果均为 122。PFGE 结果显示，巴基斯坦和泰国分离株之间存在 1 条带差异。序列型 122 是南亚出血性败血症（HS）病例的主要相关特征。该研究支持使用 PFGE 进行短期流行病学研究和 MLST 进行长期流行病学研究的概念。
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