Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA.
Exp Mol Pathol. 2011 Apr;90(2):226-30. doi: 10.1016/j.yexmp.2011.01.003. Epub 2011 Jan 18.
Leukemia/lymphoma related factor (LRF), also known as Pokemon, is a protein that belongs to the POK family of transcriptional repressors. It has an oncogenic role in many different solid tumors. In this study, the expression of LRF was evaluated in benign prostate hyperplastic (BPH) and prostate cancer (PC) tissues. The functional expression of LRF was studied using multiple cellular and molecular methods including RT-PCR, western blotting, immunohistochemistry, and immunofluorescence. Paraffin-embedded human tissues of BPH and PC were used to examine LRF expression. Histological staining of the BPH and PC tissue sections revealed nuclear expression of LRF with minimal expression in the surrounding stroma. The semi-quantitative RT-PCR and western immunoblot analyses demonstrated significantly higher mRNA transcripts and protein expression in PC than BPH. High expression of LRF suggests that it may have a potential role in the pathogenesis of both BPH and prostate cancer. Further studies will help elucidate the mechanisms and signaling pathways that LRF may follow in the pathogenesis of prostate carcinoma.
白血病/淋巴瘤相关因子（LRF），也称为 Pokemon，是一种属于 POK 家族的转录抑制剂。它在许多不同的实体肿瘤中具有致癌作用。在这项研究中，评估了良性前列腺增生（BPH）和前列腺癌（PC）组织中 LRF 的表达。使用多种细胞和分子方法，包括 RT-PCR、western blotting、免疫组织化学和免疫荧光，研究了 LRF 的功能表达。使用石蜡包埋的人 BPH 和 PC 组织检查 LRF 表达。BPH 和 PC 组织切片的组织学染色显示核表达 LRF，周围基质表达最小。半定量 RT-PCR 和 western 免疫印迹分析显示 PC 中 mRNA 转录本和蛋白表达明显高于 BPH。LRF 的高表达表明它可能在 BPH 和前列腺癌的发病机制中具有潜在作用。进一步的研究将有助于阐明 LRF 在前列腺癌发病机制中可能遵循的机制和信号通路。
