Segawa Yoshihiro, Yoshimura Rikio, Hase Taro, Nakatani Tatsuya, Wada Seiji, Kawahito Yutaka, Kishimoto Taketoshi, Sano Hajime
Department of Urology, Osaka City University Medical School, 1-4-3 Asahi-machi, Abenoku, Osaka 545-8585, Japan.
Prostate. 2002 May 1;51(2):108-16. doi: 10.1002/pros.10058.
Recent studies have demonstrated that peroxisome proliferator activator-receptors (PPAR)-gamma is expressed in some cancer cells such as breast, lung, and gastric cancer, and its ligand induces growth arrest of these cancer cells through apoptosis. However, the expression and localization of PPARs in prostate have not been examined. In this study, PPARs expression was investigated in human prostate cancer (PC), prostatic intraepithelial neoplasia (PIN), benign prostatic hyperplasia (BPH), and normal prostate (NP) tissues.
Tumor specimens were obtained from 156 patients with PC, 15 with PIN, 20 with BPH, and 12 patients with NP tissues. The expressions were investigated by RT-PCR and immunohistochemical methods.
Immunoreactive PPAR-alpha and -beta were significantly apparent in PC tissues. Marked expressions of PPAR-alpha and -beta were also detected in PIN, BPH, and NP groups. However, very weak or no expression of immunoreactive PPAR-gamma was found in BPH and NP cases. In contrast, we found significant expression of immunoreactive PPAR-gamma in cancer cells in PC group and in PIN group.
Our results demonstrated that PPAR-gamma is induced in PC, and suggest that PPAR-gamma ligands may mediate its own potent antiproliferative effect against PC cells through differentiation.
近期研究表明，过氧化物酶体增殖物激活受体（PPAR）-γ在某些癌细胞中表达，如乳腺癌、肺癌和胃癌细胞，其配体通过诱导凋亡使这些癌细胞生长停滞。然而，PPARs在前列腺中的表达及定位尚未得到研究。在本研究中，对人前列腺癌（PC）、前列腺上皮内瘤变（PIN）、良性前列腺增生（BPH）及正常前列腺（NP）组织中的PPARs表达进行了研究。
从156例PC患者、15例PIN患者、20例BPH患者及12例NP组织患者获取肿瘤标本。通过逆转录-聚合酶链反应（RT-PCR）及免疫组织化学方法研究其表达情况。
免疫反应性PPAR-α和-β在PC组织中明显可见。在PIN、BPH及NP组中也检测到PPAR-α和-β的显著表达。然而，在BPH及NP病例中，免疫反应性PPAR-γ表达非常弱或无表达。相反，在PC组和PIN组的癌细胞中发现免疫反应性PPAR-γ有显著表达。
我们的结果表明PC中诱导了PPAR-γ表达，并提示PPAR-γ配体可能通过分化介导其对PC细胞强大的抗增殖作用。
