Geng Y Q, Jiang R H, Li J
Department of Biology, Nankai University, Tianjin.
Yi Chuan Xue Bao. 1990;17(5):398-404.
The plasmid pAmy411 carrying thermostable alpha-amylase gene has been transduced into B. subtilis BF7658 by bacteriophage PBS1. The transduction frequency was 10(-9) transductants per PFU. The stability of plasmid pAmy411 in B. subtilis BF7658 was lower than that in B. subtilis AS1.1176, whereas the copy numbers were double than that in B. subtilis AS1.1176. The expressive level of thermostable alpha-amylase gene was about 6 times higher than that in B. subtilis AS1.1176.
携带耐热α-淀粉酶基因的质粒pAmy411已通过噬菌体PBS1转导至枯草芽孢杆菌BF7658中。转导频率为每噬菌斑形成单位10(-9)个转导子。质粒pAmy411在枯草芽孢杆菌BF7658中的稳定性低于在枯草芽孢杆菌AS1.1176中的稳定性，而拷贝数是枯草芽孢杆菌AS1.1176中的两倍。耐热α-淀粉酶基因的表达水平比枯草芽孢杆菌AS1.1176中的高约6倍。
