Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland.
Departamento de Sanidad Animal, Facultad de Veterinaria and VISAVET (Centro de Vigilancia Sanitaria Veterinaria), Universidad Complutense de Madrid, 28040 Madrid, Spain.
J Med Microbiol. 2011 Sep;60(Pt 9):1306-1311. doi: 10.1099/jmm.0.024026-0. Epub 2011 Jan 20.
We characterized 17 clinical isolates of Klebsiella pneumoniae producing 16S rRNA methylase ArmA. The isolates originated in Poland from 2002 to May 2010 and encompassed four XbaI-PFGE clusters. All the isolates were resistant to amikacin, gentamicin and kanamycin (MIC range: 256-1024 mg l(-1)) and carried the armA gene on a large plasmid of approximately 90 or 130 kb in 15 and 2 isolates, respectively. The armA gene was found in a ~10 kb ClaI restriction fragment of the large plasmid and was flanked by the same elements as in Tn1548. All the isolates carried the bla(CTX-M) gene for a CTX-M-type extended-spectrum β-lactamase. Our results show that ArmA has disseminated horizontally among K. pneumoniae isolates in Poland on the ~90 kb plasmid of the pCTX-M3 family.
我们对产生 16S rRNA 甲基化酶 ArmA 的 17 株肺炎克雷伯菌临床分离株进行了特征分析。这些分离株源自 2002 年至 2010 年 5 月期间的波兰，包含 4 个 XbaI-PFGE 聚类。所有分离株均对阿米卡星、庆大霉素和卡那霉素（MIC 范围：256-1024mg l(-1))）耐药，并且在 15 株和 2 株分离株中分别携带约 90 或 130kb 的大型质粒上的 armA 基因。armA 基因位于大型质粒的约 10kb ClaI 限制片段中，两侧是与 Tn1548 相同的元件。所有分离株均携带 bla(CTX-M)基因，用于 CTX-M 型超广谱β-内酰胺酶。我们的研究结果表明，在波兰，肺炎克雷伯菌分离株中 ArmA 已通过 pCTX-M3 家族的约 90kb 质粒在水平方向上传播。
