Division of Marine Environment and Bioscience, College of Ocean Science and Technology, Korea Maritime University, Busan, Korea.
Lab Anim (NY). 2011 Feb;40(2):45-51. doi: 10.1038/laban0211-45.
Fish may be anesthetized for various experimental and practical purposes, primarily to immobilize them in order to facilitate handling. Marine medaka (Oryzias dancena) is a teleost fish used in marine ecotoxicology studies. Despite the importance of anesthesia in handling experimental fish, the effects of anesthesia in marine medaka have not yet been investigated. In this study, the authors evaluated the anesthetic effects (time required for anesthesia to take effect and recovery time) of two anesthetic agents, clove oil and lidocaine-HCl, on marine medaka. They anesthetized fish at different water temperatures (23 °C, 26 °C and 29 °C) and using different concentrations of clove oil (50 ppm, 75 ppm, 100 ppm, 125 ppm, 150 ppm and 175 ppm) or lidocaine-HCl (300 ppm, 400 ppm, 500 ppm, 600 ppm, 700 ppm and 800 ppm). The time required for anesthesia to take effect decreased significantly as both anesthetic concentration and water temperature increased for both clove oil and lidocaine-HCl. To anesthetize marine medaka within approximately 1 min, the optimal concentrations for clove oil were 125 ppm at 23 °C, 100 ppm at 26 °C and 75 ppm at 29 °C and for lidocaine-HCl were 800 ppm at 23 °C and 700 ppm at both 26 °C and 29 °C. The authors also compared anesthetic effects in marine medaka of different sizes. Both anesthetic exposure time and recovery time were significantly shorter for smaller fish than for larger fish. These results provide a useful foundation for the laboratory handling of marine medaka.
鱼类可能会因各种实验和实际目的而被麻醉，主要是为了使它们固定不动，以便于处理。海洋稻（Oryzias dancena）是一种用于海洋生态毒理学研究的硬骨鱼。尽管麻醉在处理实验鱼类方面很重要，但海洋稻的麻醉效果尚未得到研究。在这项研究中，作者评估了两种麻醉剂（丁香油和盐酸利多卡因）对海洋稻的麻醉效果（麻醉生效所需时间和恢复时间）。他们在不同的水温（23°C、26°C 和 29°C）下，用不同浓度的丁香油（50ppm、75ppm、100ppm、125ppm、150ppm 和 175ppm）或盐酸利多卡因（300ppm、400ppm、500ppm、600ppm、700ppm 和 800ppm）麻醉鱼类。对于丁香油和盐酸利多卡因，麻醉生效所需的时间都随着麻醉剂浓度和水温的升高而显著缩短。为了在大约 1 分钟内麻醉海洋稻，丁香油的最佳浓度分别为 23°C 时 125ppm、26°C 时 100ppm 和 29°C 时 75ppm，盐酸利多卡因的最佳浓度分别为 23°C 时 800ppm 和 26°C 和 29°C 时 700ppm。作者还比较了不同大小的海洋稻的麻醉效果。对于较小的鱼类，麻醉暴露时间和恢复时间都明显短于较大的鱼类。这些结果为海洋稻的实验室处理提供了有用的基础。
