Park In-Seok
Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime & Ocean University, Busan 49112, Korea.
Dev Reprod. 2019 Mar;23(1):21-34. doi: 10.12717/DR.2019.23.1.021. Epub 2019 Mar 31.
The effects of the anaesthetic agents, clove oil and mixture of clove oil with lidocaine-HCl were evaluated on river puffer, and tiger puffer, . Anaesthesia times of clove oil were affected by water temperature (20℃, 24℃, and 28℃) and salinity (10, 20, and 30 ppt). Anaesthesia times of mixed samples were significantly similar with regard to exposure and recovery times, and all samples satisfied anaesthesia criteria (exposure time within 3 min and recovery time within 5 min) under the various temperatures and salinities, and the lowest to highest concentration of anaesthetics (<0.05). Both species river puffer and tiger puffer had short exposure time with a high anaesthesia dose, high temperature (28℃) and intermediate salinity (20 ppt), and were highly affected by temperature and salinity (<0.05). The mixed anaesthetics had rapid exposure times and long recovery times in contrast to the effects of clove oil. Cortisol concentrations under the conditions of various clove oil dosages, salinity, and temperature for both species increased until 12 hrs after recovery from anaesthesia (<0.05). After 12 hrs, cortisol concentrations decreased until after 48 hrs (<0.05). During the simulated transportation of both species, control and sedated clove oil groups (5 ppm) were measured for water parameters, dissolved oxygen (DO), CO, respiratory frequency, NH , and pH for 6 hrs in 1 hr intervals. Water parameters of sedated groups and controls were significantly different after 2 hrs (<0.05).
评估了麻醉剂丁香油以及丁香油与盐酸利多卡因的混合物对河鲀和虎鲀的影响。丁香油的麻醉时间受水温（20℃、24℃和28℃）和盐度（10、20和30ppt）的影响。混合样本的麻醉时间在暴露和恢复时间方面显著相似，并且在各种温度和盐度以及最低至最高麻醉剂浓度（<0.05）下，所有样本均满足麻醉标准（暴露时间在3分钟内且恢复时间在5分钟内）。河鲀和虎鲀这两个物种在高麻醉剂量、高温（28℃）和中等盐度（20ppt）下暴露时间较短，并且受温度和盐度的影响很大（<0.05）。与丁香油的作用相比，混合麻醉剂的暴露时间快而恢复时间长。两种物种在各种丁香油剂量、盐度和温度条件下，皮质醇浓度在从麻醉恢复后直至12小时都升高（<0.05）。12小时后，皮质醇浓度下降直至48小时后（<0.05）。在两种物种的模拟运输过程中，对对照组和用丁香油镇静的组（5ppm）每隔1小时测量6小时的水参数、溶解氧（DO）、CO、呼吸频率、NH和pH。2小时后，镇静组和对照组的水参数有显著差异（<0.05）。
