5-溴脱氧尿苷对人结肠癌细胞系中井上-梅尔尼克病毒合成的增强作用

5-Bromodeoxyuridine enhancement of Inoue-Melnick virus synthesis in human colon carcinoma cell lines.

作者信息

Inoue Y K, Nishibe Y, Kimura T

机构信息

Institute for Virus Research, Kyoto University, Japan.

出版信息

J Med Virol. 1990 Aug;31(4):249-52. doi: 10.1002/jmv.1890310402.

DOI:10.1002/jmv.1890310402

PMID:2125309

Abstract

Inoue-Melnick virus (IMV) synthesis in naturally infected human colon carcinoma cell lines can be enhanced by 5-bromodeoxyuridine (BUDR). The optimal conditions for BUDR enhancement differed between two cell lines, SW-480 and DLD-1. SW-480 cells produced 100 times more infectious IMV than DLD-1 cells by BUDR treatment. A clonal difference in IMV synthesis in SW-480 cells was also observed. The highest titer of virus was found in the culture fluid of clone H (SW-480/H) cells treated with BUDR, and a herpes-like virus was detected by negative-staining electron microscopy of concentrates of the culture fluid. One nonproducer clone (SW-480/B) was also activated to produce virus when treated with BUDR.

摘要

在自然感染的人结肠癌细胞系中，5-溴脱氧尿苷（BUDR）可增强井上-梅尔尼克病毒（IMV）的合成。SW-480和DLD-1这两种细胞系中，BUDR增强的最佳条件有所不同。通过BUDR处理，SW-480细胞产生的感染性IMV比DLD-1细胞多100倍。在SW-480细胞中也观察到IMV合成的克隆差异。在用BUDR处理的克隆H（SW-480/H）细胞的培养液中发现了最高滴度的病毒，并且通过对培养液浓缩物进行负染色电子显微镜检测到一种疱疹样病毒。一个非生产性克隆（SW-480/B）在用BUDR处理时也被激活产生病毒。