Nishibe Y, Inoue Y K, Kimura T, Hase A
Institute for Virus Research, Kyoto University, Japan.
J Med Virol. 1989 Mar;27(3):196-200. doi: 10.1002/jmv.1890270304.
The authors found remarkable enhancement of Inoue-Melnick virus (IMV) synthesis by 5-bromodeoxyuridine (BUDR) in human meningioma (MG-1) cells, a virus-producer line of type 1 IMV. Treatment with BUDR resulted in rapid and abundant synthesis of infectious IMV in MG-1 cells. The titer of the cell-associated virus in treated cells increased approximately 6.0 log 10 compared with that in untreated cells. Immunofluorescent antibody tests revealed that IMV-associated late antigen was induced by BUDR in the cytoplasm of approximately 50% of treated cells. A clonal difference was also found in the enhancement of BUDR on the IMV synthesis in MG-1 cells. The most remarkable enhancing effect of BUDR was observed in the clone C line, and a herpes-type virus was detected by negative-staining electron microscopy in the culture fluid of the clone C treated with BUDR.
作者发现，在人脑膜瘤（MG-1）细胞（1型井上-梅尔尼克病毒（IMV）的病毒产生细胞系）中，5-溴脱氧尿苷（BUDR）可显著增强IMV的合成。用BUDR处理导致MG-1细胞中快速且大量地合成有传染性的IMV。与未处理细胞相比，处理后细胞中细胞相关病毒的滴度增加了约6.0个对数10。免疫荧光抗体试验显示，约50%的处理细胞的细胞质中，BUDR诱导了IMV相关晚期抗原。在MG-1细胞中，还发现了BUDR对IMV合成增强作用的克隆差异。在克隆C系中观察到BUDR最显著的增强作用，在用BUDR处理的克隆C的培养液中，通过负染色电子显微镜检测到一种疱疹型病毒。
