Ito M, Rodriguez-Bigas M A, Evans M J, Petrelli N J
Department of Biological Resources, Roswell Park Cancer Institute, Buffalo, New York 14263.
Cancer. 1993 Mar 15;71(6):1960-3. doi: 10.1002/1097-0142(19930315)71:6<1960::aid-cncr2820710606>3.0.co;2-7.
Isolation of Inoue-Melnick virus (IMV) Type 1 from tumor tissues of patients with colorectal carcinoma in Japan was reported. A high prevalence of IMV antibodies has been found among patients with colorectal carcinoma in Buffalo, New York.
IMV was isolated from homogenates of colorectal carcinoma and other tissues, inoculated into MRC-5 human fibroblast cultures. IMV-specific cytopathic effects were detected and titrated following 7 days of incubation in a roller-tube apparatus.
IMV was isolated from all nine (100%) colorectal carcinoma tissues tested and all four (100%) normal parts of colon mucosa tissues obtained from patients with cancer. Biopsy specimens of normal colon mucosa from 10 patients without colon carcinoma were negative for IMV (0%). Average infectious titers of IMV in these tissue homogenates were 4.41 for tumor tissue and 2.55 log10 tissue culture infectious dose by 50% (TCID50)/0.1 ml for colon mucosa, respectively. Most of the isolates were the IMV intermediate type.
Our results confirmed the production of infectious IMV in colorectal carcinoma tissues, as reported recently in Japan. The role of IMV in colorectal carcinogenesis is currently unknown.
有报道称在日本从结直肠癌患者的肿瘤组织中分离出了井上-梅尔尼克病毒1型（IMV）。在纽约布法罗的结直肠癌患者中发现IMV抗体的高流行率。
从结直肠癌和其他组织的匀浆中分离出IMV，接种到MRC-5人成纤维细胞培养物中。在滚管装置中孵育7天后检测并滴定IMV特异性细胞病变效应。
在所有检测的9个（100%）结直肠癌组织以及从癌症患者获得的所有4个（100%）结肠黏膜组织正常部分中均分离出IMV。10名无结肠癌患者的正常结肠黏膜活检标本IMV检测为阴性（0%）。这些组织匀浆中IMV的平均感染滴度，肿瘤组织为4.41，结肠黏膜为2.55 log10 50%组织培养感染剂量（TCID50）/0.1 ml。大多数分离株为IMV中间型。
我们的结果证实了结直肠癌组织中产生了有传染性的IMV，正如最近在日本报道的那样。目前尚不清楚IMV在结直肠癌发生中的作用。
