Department of Chemistry & Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, P. R. China.
Chem Soc Rev. 2011 May;40(5):2508-24. doi: 10.1039/c0cs00114g. Epub 2011 Jan 21.
The application of phosphorescent heavy-metal complexes with d(6), d(8) and d(10) electron configurations for bioimaging is a new and promising research field and has been attracting increasing interest. In this critical review, we systematically evaluate the advantages of phosphorescent heavy-metal complexes as bioimaging probes, including their photophysical properties, cytotoxicity and cellular uptake mechanisms. The progress of research into the use of phosphorescent heavy-metal complexes for staining different compartments of cells, monitoring intracellular functional species, providing targeted bioimaging, two-photon bioimaging, small-animal bioimaging, multimodal bioimaging and time-resolved bioimaging is summarized. In addition, several possible future directions in this field are also discussed (133 references).
具有 d(6)、d(8) 和 d(10) 电子构型的磷光重金属配合物在生物成像中的应用是一个新的、有前途的研究领域，越来越受到关注。在这篇评论中，我们系统地评估了磷光重金属配合物作为生物成像探针的优势，包括它们的光物理性质、细胞毒性和细胞摄取机制。总结了研究利用磷光重金属配合物对细胞不同区室进行染色、监测细胞内功能物种、提供靶向生物成像、双光子生物成像、小动物生物成像、多模态生物成像和时间分辨生物成像的进展。此外，还讨论了该领域的几个可能的未来方向（引用 133 篇）。
