Yu Zhen-hai, Huang Fei, Xu Ning, Zhao Dong-mei, Hu Feng-ai, Liu Jiao, Liu Hong-fu
Department of Anatomy, Binzhou Medical University, Binzhou, P. R. China.
J Immunoassay Immunochem. 2011;32(1):47-56. doi: 10.1080/15321819.2010.538108.
This study elucidates the significance of Toll-like receptor 4 (TLR4), CD14, and nuclear factor (NF)-κB on the pathogenesis of ulcerative colitis (UC). Colonic biopsy specimens were collected from active UC and controls. The expression of TLR4, CD14, and NF-κBp 65 was analyzed by immunohistochemistry (IHC) and reverse-transcription polymerase chain reaction (RT-PCR). In UC, disease activity index (DAI) and pathological grade were classified according to the Powell-Tuck grade system and Truelove-Richards system, respectively. Fifty-six UC cases and 56 controls entered the investigation. IHC and RT-PCR revealed a significant increase of TLR4, CD14, and NF-κBp 65 antigen expression in colonic mucosa of UC compared with colonic mucosa of controls (p < .001). In UC, TLR4, CD14, and NF-κBp 65 expression were positively related to DAI (r = .873, p < .001; r = .576, p < .001; r = .747, p < .001 receptively). NF-κBp65 significantly correlated with TLR4 and CD14 (r = .669, p < .001; r = .576, p < .001, receptively). TLR4, CD14, and NF-κBp65 were positively related to pathological classification in UC (p < .01). Thus, TLR4, CD14, and NF-κBp65 were upregulated significantly in UC, to an extent that reflects the degree of inflammation and thereby might contribute to the occurrence and development of UC.
本研究阐明了Toll样受体4（TLR4）、CD14和核因子（NF）-κB在溃疡性结肠炎（UC）发病机制中的意义。从活动期UC患者和对照组收集结肠活检标本。通过免疫组织化学（IHC）和逆转录聚合酶链反应（RT-PCR）分析TLR4、CD14和NF-κBp 65的表达。在UC中，疾病活动指数（DAI）和病理分级分别根据Powell-Tuck分级系统和Truelove-Richards系统进行分类。56例UC病例和56例对照纳入研究。IHC和RT-PCR显示，与对照组结肠黏膜相比，UC患者结肠黏膜中TLR4、CD14和NF-κBp 65抗原表达显著增加（p < 0.001）。在UC中，TLR4、CD14和NF-κBp 65表达与DAI呈正相关（r = 0.873，p < 0.001；r = 0.576，p < 0.001；r = 0.747，p < 0.001）。NF-κBp65与TLR4和CD14显著相关（r = 0.669，p < 0.001；r = 0.576，p < 0.001）。TLR4、CD14和NF-κBp65与UC的病理分类呈正相关（p < 0.01）。因此，TLR4、CD14和NF-κBp65在UC中显著上调，上调程度反映了炎症程度，从而可能促进UC的发生和发展。
