Körmendy B
Veterinary and Food Control Service, Central Veterinary Institute, Budapest, Hungary.
Acta Microbiol Hung. 1990;37(2):219-22.
In a beef herd of 230 cows kept on pasture and infected with Mycobacterium paratuberculosis, faecal samples were tested microscopically and the blood serum by the complement fixation and the immunodiffusion test. Intradermal tests were carried out simultaneously with bovine and avian tuberculin as well as with two dilutions (0.86 mg/ml and 0.50 mg/ml tuberculoprotein) of paratuberculin. In the tested herd 35.2% of the cows gave positive faecal tests while 10 and 9.1% were positive in the agar gel immunodiffusion (AGID) and complement fixation test (CFT), respectively. The allergens showed wide variations, but as a whole they were positive in 58.1% of the faecal positive, and in 60.9 and 76.2% of the AGID and CFT positive ones, respectively. The allergic tests were positive in 54.4, 53.7 and 52.2% of the faecal, AGID and CFT negative cows, respectively. The prognosis of such herds is disadvantageous. Elimination of infection can only be expected if after improvement of hygienic circumstances the diseased animals are continuously culled and a new breeding stock reared in isolation.
在一个饲养在牧场且感染了副结核分枝杆菌的230头母牛的牛群中，对粪便样本进行了显微镜检查，并通过补体结合试验和免疫扩散试验检测了血清。同时用牛结核菌素、禽结核菌素以及两种稀释度（0.86毫克/毫升和0.50毫克/毫升结核蛋白）的副结核菌素进行皮内试验。在所检测的牛群中，35.2%的母牛粪便检测呈阳性，而琼脂凝胶免疫扩散试验（AGID）和补体结合试验（CFT）的阳性率分别为10%和9.1%。过敏原显示出很大差异，但总体而言，它们在58.1%的粪便阳性牛、60.9%的AGID阳性牛和76.2%的CFT阳性牛中呈阳性。过敏试验在粪便、AGID和CFT阴性的母牛中阳性率分别为54.4%、53.7%和52.2%。此类牛群的预后不利。只有在改善卫生条件后持续淘汰患病动物并隔离饲养新的种畜，才有望消除感染。
