Colgrove G S, Thoen C O, Blackburn B O, Murphy C D
U.S.D.A., Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, Ames, IA 50010.
Vet Microbiol. 1989 Feb;19(2):183-7. doi: 10.1016/0378-1135(89)90083-7.
Feces and blood were collected from cattle in 13 herds known to be infected with Mycobacterium paratuberculosis to evaluate a complement-fixation (CF) test, an agar gel immunodiffusion (AGID) test and an enzyme-linked immunosorbent assay (ELISA) for the serologic diagnosis of paratuberculosis. M. paratuberculosis was isolated from the feces of 36 of 192 cattle examined. Twenty-three culture-positive animals had CF test titers regarded as suspect or positive, 10 were positive by the AGID test and 34 were suspect or positive by the ELISA. Of the 156 culture-negative animals, the CF test agreed on 136, the ELISA on 129 and the AGID on 151.
从已知感染副结核分枝杆菌的13个牛群中采集粪便和血液样本，以评估补体结合（CF）试验、琼脂凝胶免疫扩散（AGID）试验和酶联免疫吸附测定（ELISA）用于副结核病的血清学诊断。在所检测的192头牛中，从36头牛的粪便中分离出了副结核分枝杆菌。23头培养阳性的动物CF试验效价被视为可疑或阳性，10头AGID试验呈阳性，34头ELISA试验呈可疑或阳性。在156头培养阴性的动物中，CF试验与136头结果一致，ELISA与129头一致，AGID与151头一致。
