Saghiri Mohammad Ali, Asgar Kamal, Daliri Morteza, Lotfi Mehrdad, Delvarani Abbas, Mehrvarzfar Payman, Karamifar Kasra
Department of Dental Material, Dental Branch, Islamic Azad University, Tehran, Iran.
Scanning. 2010 Nov-Dec;32(6):369-74. doi: 10.1002/sca.20209. Epub 2010 Nov 9.
Some techniques and instruments like stereomicroscopy and confocal microscopy used for observing neural cells are too complicated and dependent on preparation and cell fixation methods. This may question the results of these methods. Though, we have used scanning electron microscopy on replicated specimens to observe p19 neural cells and their cellular extensions. This manuscript has shown the feasibility of using replica (indirect) method instead of direct methods for observing morphological characteristics of this high sensitive cell line. As neural cells are very sensitive to fixation solutions and processes, we have used replica mode and observed neural cells with a novel indirect method. We have used replica mode in this study to indirectly and noninvasively evaluate the state of p19 neural cells and their cellular extensions.
一些用于观察神经细胞的技术和仪器，如体视显微镜和共聚焦显微镜，过于复杂，且依赖于制备和细胞固定方法。这可能会质疑这些方法的结果。不过，我们已在复制标本上使用扫描电子显微镜来观察p19神经细胞及其细胞突起。本手稿展示了使用复制（间接）方法而非直接方法来观察这种高敏感性细胞系形态特征的可行性。由于神经细胞对固定液和固定过程非常敏感，我们采用了复制模式并用一种新颖的间接方法观察神经细胞。在本研究中，我们使用复制模式来间接且无创地评估p19神经细胞及其细胞突起的状态。
