Karpov P A, Raevskiĭ A V, Blium Ia B
Tsitol Genet. 2010 Nov-Dec;44(6):57-69.
Fourteen plant homologs of animal, yeast and myxomycetes spindle assembly checkpoint protein kinases were identified bioinformatically. It was shown that the closest plant homologues of the BUB1 protein kinases are unknown proteins XP_002274770.1 (CBI21878.1) from Vitis vinifera, EEC82122.1 from Oryza sativa Indica, EEE67244.1 from O. sativa Japonica, EEF44403.1 from Ricinus communis and CAL57156.1 from Ostreococcus tauri. The reconstruction and analysis of spatial structures of the EEC82122.1, EEE67244.1 and XP_002274770.1 (CBI21878.1), catalytic domains confirmed their conformity to spindle assembly checkpoint protein kinases BUB1.
通过生物信息学方法鉴定出了14种与动物、酵母和黏菌纺锤体组装检查点蛋白激酶同源的植物蛋白。结果表明，BUB1蛋白激酶在植物中最相近的同源物是来自葡萄的未知蛋白XP_002274770.1（CBI21878.1）、来自籼稻的EEC82122.1、来自粳稻的EEE67244.1、来自蓖麻的EEF44403.1以及来自塔胞藻的CAL57156.1。对EEC82122.1、EEE67244.1和XP_002274770.1（CBI21878.1）催化结构域的空间结构进行重建和分析，证实它们与纺锤体组装检查点蛋白激酶BUB1相符。
