Institute of Nutraceuticals and Functionnal Foods (INAF).
J Agric Food Chem. 2011 Mar 9;59(5):1784-92. doi: 10.1021/jf103739m. Epub 2011 Jan 21.
Electrodialysis with ultrafiltration membrane (EDUF) is a technology based on the separation of molecules according to their charge and molecular mass. Some works have already successfully demonstrated the recovery of bioactive peptide fractions. However, the impact of ultrafiltration membrane (UFM) material, used in the EDUF system, on the peptide migration has never been studied. Consequently, the objectives of this work were (1) to evaluate the effect of two different UFM materials on the selective separation of peptides from a snow crab byproduct hydrolysate by electrodialysis with ultrafiltration membranes and (2) to determine the effect of UFM material on their potential fouling by peptides. It appeared that, after 6 h of EDUF separation using polyether sulfone (PES) and cellulose acetate (CA) UFM, peptides with low molecular weights ranging from 300 to 700 Da represented the most abundant population in the KCl1 (compartment located near the anode for the recovery of anionic/acid peptide fractions) and KCl2 (compartment located near the cathode for the recovery of cationic/basic peptide fractions) permeates. Peptides with molecular weights ranging from 700 to 900 Da did not migrate during the EDUF treatment. Moreover, only CA UFM allowed the recovery of high molecular weight molecules (900-20000 Da) in both KCl compartments. Peptides desorbed from PES and CA UFM after 6 h of EDUF separation had low molecular weights and belonged mainly to the 600-700 Da molecular weight range. These peptides represented a low proportion of the peptides initially present in the snow crab byproduct hydrolysate with individual molecular weight range proportions from 1.52 ± 0.31 to 10.2 ± 2.32%.
电渗析与超滤膜（EDUF）是一种根据分子的电荷和分子量进行分离的技术。已经有一些工作成功地证明了生物活性肽段的回收。然而，用于 EDUF 系统的超滤膜（UFM）材料对肽迁移的影响从未被研究过。因此，本工作的目的是：（1）评估两种不同的 UFM 材料对雪蟹副产物水解物通过电渗析与超滤膜选择性分离肽的影响；（2）确定 UFM 材料对其潜在污染的影响。结果表明，在使用聚醚砜（PES）和醋酸纤维素（CA）UFM 进行 6 小时 EDUF 分离后，分子量在 300 到 700 Da 之间的低分子量肽是 KCl1（位于阳极附近，用于回收阴离子/酸性肽段）和 KCl2（位于阴极附近，用于回收阳离子/碱性肽段）透过液中最丰富的肽段。分子量在 700 到 900 Da 之间的肽段在 EDUF 处理过程中没有迁移。此外，只有 CA UFM 允许在两个 KCl 隔室中回收高分子量分子（900-20000 Da）。在 6 小时的 EDUF 分离后，从 PES 和 CA UFM 中解吸的肽具有低分子量，主要属于 600-700 Da 分子量范围。这些肽段代表了最初存在于雪蟹副产物水解物中的肽段的一小部分，其各自的分子量范围比例为 1.52±0.31 至 10.2±2.32%。
