Department of Food Sciences and Laboratory of Food Processing and Electromembrane Processes (LTAPEM), Université Laval, Quebec, QC G1V 0A6, Canada.
Institute of Nutrition and Functional Foods (INAF), Université Laval, Quebec, QC G1V 0A6, Canada.
Int J Mol Sci. 2019 Apr 20;20(8):1939. doi: 10.3390/ijms20081939.
The valorization of by-products from natural organic sources is an international priority to respond to environmental and economic challenges. In this context, electrodialysis with filtration membrane (EDFM), a green and ultra-selective process, was used to separate peptides from salmon frame protein hydrolysate. For the first time, the simultaneous separation of peptides by three ultrafiltration membranes of different molecular-weight exclusion limits (50, 20, and 5 kDa) stacked in an electrodialysis system, allowed for the generation of specific cationic and anionic fractions with different molecular weight profiles and bioactivity responses. Significant decreases in peptide recovery, yield, and molecular weight (MW) range were observed in the recovery compartments depending on whether peptides had to cross one, two, or three ultrafiltration membranes. Moreover, the Cationic Recovery Compartment 1 fraction demonstrated the highest increase (42%) in glucose uptake on L6 muscle cells. While, in the anionic configuration, both Anionic Recovery Compartment 2 and Anionic Recovery Compartment 3 fractions presented a glucose uptake response in basal condition similar to the insulin control. Furthermore, Cationic Recovery Compartment 3 was found to contain inhibitory peptides. Finally, LC-MS analyses of the bioassay-guided bioactive fractions allowed us to identify 11 peptides from salmon by-products that are potentially responsible for the glucose uptake improvement.
从天然有机资源中副产物的增值利用是应对环境和经济挑战的国际优先事项。在此背景下，采用绿色、超高选择性的电渗析与过滤膜（EDFM）工艺，从三文鱼下脚料蛋白水解物中分离肽段。这是首次在电渗析系统中采用三种不同截留分子量的超滤膜（50、20 和 5 kDa）进行肽的同步分离，从而得到具有不同分子量分布和生物活性响应的特定阳离子和阴离子级分。根据肽是否需要穿过一个、两个或三个超滤膜，在回收隔室中观察到肽回收率、产率和分子量（MW）范围显著降低。此外，阳离子回收隔室 1 级分在 L6 肌肉细胞上表现出最高的葡萄糖摄取增加（42%）。而在阴离子构型中，阴离子回收隔室 2 和阴离子回收隔室 3 级分在基础条件下的葡萄糖摄取反应与胰岛素对照相似。此外，发现阳离子回收隔室 3 含有抑制肽。最后，通过对生物活性级分的生物测定指导的 LC-MS 分析，从三文鱼副产物中鉴定出 11 种可能负责改善葡萄糖摄取的肽。
