Doyen Alain, Saucier Linda, Beaulieu Lucie, Pouliot Yves, Bazinet Laurent
Institute of Nutraceuticals and Functional Foods (INAF), Université Laval, Québec, QC, Canada G1V 0A6; Department of Food Science and Nutrition, Université Laval, Québec, QC, Canada G1V 0A6.
Institute of Nutraceuticals and Functional Foods (INAF), Université Laval, Québec, QC, Canada G1V 0A6; Department of Animal Sciences, Université Laval, Québec, QC, Canada G1V 0A6.
Food Chem. 2012 Jun 1;132(3):1177-1184. doi: 10.1016/j.foodchem.2011.11.059. Epub 2011 Nov 18.
Recently, a snow crab by-products hydrolysate has demonstrated antibacterial properties due to a peptide with a molecular weight of about 800Da, but only at high concentration. Consequently, peptide hydrolysate has been fractionated to obtain peptides in a more purified form. The aim of this work was to separate a snow crab by-products hydrolysate by electrodialysis with ultrafiltration membranes (EDUF). EDUF, which allows separation of molecules according to their charges and molecular weights, was used to recover and concentrate the active antibacterial fraction. Two different ultrafiltration membranes (20 and 50kDa) and two electrical field strengths (2 and 14V/cm) were used as separation parameters. After EDUF separation, the 300-600Da peptide molecular weight range was the most recovered with an abundance of 94%. Moreover, fractionation at 14V/cm with ultrafiltration membranes of 50kDa allowed the recovery of an anionic fraction which showed antibacterial properties on Escherichia coli ATCC 25922 and Listeria innocua HPB 13.
最近，一种雪蟹副产物水解物由于一种分子量约为800Da的肽而表现出抗菌特性，但仅在高浓度时如此。因此，已对肽水解物进行分级分离以获得更纯化形式的肽。这项工作的目的是通过带有超滤膜的电渗析（EDUF）分离雪蟹副产物水解物。EDUF能够根据分子的电荷和分子量进行分离，被用于回收和浓缩活性抗菌组分。使用两种不同的超滤膜（20和50kDa）以及两种电场强度（2和14V/cm）作为分离参数。经过EDUF分离后，300 - 600Da的肽分子量范围回收率最高，达到94%。此外，使用50kDa的超滤膜在14V/cm下进行分级分离能够回收一种阴离子组分，该组分对大肠杆菌ATCC 25922和无害李斯特菌HPB 13表现出抗菌特性。
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