Institut de Recherche pour le Développement, Society and Health Department, MIVEGEC, University of Montpellier - CNRS 5290 - IRD 224, Representation in Bolivia, Av Hernando Siles N°5290, CP 9214, La Paz, Bolivia.
Infect Genet Evol. 2011 Jul;11(5):1155-8. doi: 10.1016/j.meegid.2010.11.013. Epub 2011 Jan 19.
In order to validate a rapid typing of Trypanosoma cruzi DTUs, the miniexon multiplex PCR was tested for the first time, on a large and diversified sample of 70 strains belonging to all current DTUs (TcI to TcVI). Three DTU groups have been distinguished by specific PCR molecular weight, TcI (200bp), TcII, V, VI (250bp) and TcIII and IV (150bp) with no incorrect grouping. These groups are epidemiologically and genetically relevant; moreover the method is easy and cheap and allows direct identification of parasites from triatomine faeces.
为了验证对克氏锥虫 DTU 的快速分型，首次对来自所有当前 DTU（TcI 至 TcVI）的 70 株大而多样的菌株的小外显子多重 PCR 进行了测试。通过特定的 PCR 分子量将三个 DTU 组区分开来，TcI（200bp）、TcII、V、VI（250bp）和 TcIII 和 IV（150bp），没有错误分组。这些组在流行病学和遗传学上是相关的；此外，该方法简单且廉价，并允许直接从三锥虫粪便中鉴定寄生虫。
