控制枯草芽孢杆菌利用木聚糖的基因。
Genes controlling xylan utilization by Bacillus subtilis.
作者信息
Roncero M I
出版信息
J Bacteriol. 1983 Oct;156(1):257-63. doi: 10.1128/jb.156.1.257-263.1983.
Abstract
Eight mutants of Bacillus subtilis deficient in xylan utilization were isolated and characterized genetically and biochemically. Each mutant was obtained independently after nitrosoguanidine mutagenesis. All of the analyzed mutations were shown to be linked. Reciprocal transformation crosses revealed the existence of two genes controlling xylan utilization which have been designated xynA and xynB. Available data have indicated that these two genes code for two xylan-degrading enzymes existing in the wild-type strains, an extracellular beta-xylanase (xynA) and a cell-associated beta-xylosidase (xynB).
摘要
分离出8株枯草芽孢杆菌木聚糖利用缺陷型突变体，并对其进行了遗传和生化特性分析。每个突变体都是在亚硝基胍诱变后独立获得的。所有分析的突变都显示是连锁的。相互转化杂交揭示了存在两个控制木聚糖利用的基因，分别命名为xynA和xynB。现有数据表明，这两个基因编码野生型菌株中存在的两种木聚糖降解酶，一种胞外β-木聚糖酶（xynA）和一种细胞相关的β-木糖苷酶（xynB）。
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