Tomita Y, Lieberman B Y, Cathcart M K
Department of Immunology, Cleveland Clinic Foundation, Ohio.
Immunobiology. 1990 Aug;181(1):64-83. doi: 10.1016/S0171-2985(11)80166-X.
We have made a human thymus cell hybridoma that secretes an immunosuppressive monoclonal lymphokine, referred to as hybridoma suppressor factor (HSF). This factor modulates the function of CD4+ cells suppressing their IL-2 production and suppressing PWM-induced B cell differentiation into Ig producing cells. Here we have examined the effect of HSF on the generation of T cell-derived lymphokines that regulate B cell growth and differentiation as well as the expression of other proteins involved in the control of T cell growth i.e., the p55 chain of the IL-2R and the transferrin receptor (TFR). HSF suppressed IFN-gamma activity produced by mitogen-stimulated PBMC without affecting the generation of lymphokines responsible for BCGF and BCDF activities. Additionally, HSF did not inhibit the expression of either IL-2R (p55) or TFR by activated T cells in spite of causing the suppression of IL-2 production. This evidence was further supported by experiments in which HSF selectively suppressed the accumulation of IL-2 mRNA without affecting IL-2R (p55) mRNA expression in mitogen-stimulated PBMC. The selective action of HSF may help to clarify the regulatory mechanisms involved in lymphokine gene expression as well as provide a way by which immune responses involved in autoimmunity and transplant rejection may be interrupted.
我们制备了一种人胸腺细胞杂交瘤，它能分泌一种免疫抑制性单克隆淋巴因子，称为杂交瘤抑制因子（HSF）。该因子可调节CD4 +细胞的功能，抑制其IL-2的产生，并抑制PWM诱导的B细胞分化为产生Ig的细胞。在此，我们研究了HSF对调节B细胞生长和分化的T细胞衍生淋巴因子的产生以及参与控制T细胞生长的其他蛋白质（即IL-2R的p55链和转铁蛋白受体（TFR））表达的影响。HSF抑制了有丝分裂原刺激的PBMC产生的IFN-γ活性，而不影响负责BCGF和BCDF活性的淋巴因子的产生。此外，尽管HSF抑制了IL-2的产生，但它并未抑制活化T细胞对IL-2R（p55）或TFR的表达。有丝分裂原刺激的PBMC实验进一步支持了这一证据，其中HSF选择性地抑制了IL-2 mRNA的积累，而不影响IL-2R（p55）mRNA的表达。HSF的选择性作用可能有助于阐明淋巴因子基因表达所涉及的调节机制，并提供一种中断自身免疫和移植排斥中免疫反应的方法。
