Dipartimento Farmaco-chimico, Università degli Studi di Messina, Messina, Italy.
J Chromatogr A. 2011 May 27;1218(21):3140-5. doi: 10.1016/j.chroma.2010.11.082. Epub 2010 Dec 7.
The present investigation is focused on a simple flow modulator (FM), for comprehensive two-dimensional gas chromatography (GC×GC). The interface is stable at high temperatures, and consists of a metallic disc (located inside the GC oven) with seven ports, which are connected to an auxiliary pressure source via two branches, to the first and second dimension, to a waste branch (linked to a needle valve) and to an exchangeable modulation loop (2 ports). The ports are connected via micro-channels, etched on one of the inner surfaces of the disc. Modulation is achieved using a two-way electrovalve, connected on one side to the additional pressure source, and to the two metal branches, on the other. An FM enantio-GC×polar-GC method (using a flame ionization detector) was optimized (a 40-μL loop was employed), for the analysis of essential oils. As an example, an application on spearmint oil is shown; the method herein proposed was subjected to validation. Finally, an FM GC×GC diesel experiment was carried out, using an apolar-polar column combination, to demonstrate the effectiveness of the modulator in the analysis of a totally different sample-type.
本研究专注于一种简单的流量调制器 (FM)，用于全二维气相色谱 (GC×GC)。该接口在高温下稳定，由一个带有七个端口的金属盘（位于 GC 炉内）组成，通过两个分支与辅助压力源连接，分别连接到第一维和第二维、废物分支（与针阀相连）和可交换的调制环（2 个端口）。端口通过微通道连接，这些微通道蚀刻在圆盘的一个内表面上。调制是通过一个双向电磁阀实现的，该电磁阀一侧连接到附加压力源，另一侧连接到两个金属分支。采用火焰离子化检测器，优化了 FM 对映体-GC×极性-GC 方法（使用 40 μL 环），用于分析精油。作为一个例子，展示了对薄荷油的应用；本文提出的方法进行了验证。最后，使用非极性-极性柱组合进行了 FM GC×GC 柴油实验，以证明调制器在分析完全不同样品类型时的有效性。
