Oakland University, Department of Chemistry, Rochester, MI 48309, USA.
J Chromatogr A. 2012 Sep 14;1255:24-37. doi: 10.1016/j.chroma.2012.01.027. Epub 2012 Jan 18.
The continued development of flow-controlled two-dimensional gas chromatography (2-D GC) is reviewed, with a special emphasis on results published from 2001 through 2011. Heart-cutting 2-D GC continues to be used for isolating selected components in complex mixtures. The programmable and highly precise flows and temperatures produced by modern gas chromatographs have made it easier to selectively transfer analytes to the secondary column and to backflush unwanted components from the primary column. Several new Deans switch interfaces for performing heart-cutting 2-D GC have been introduced, with most attention given to devices that integrate the flow connections into a single unit. Heart-cutting 2-D GC has been used to isolate analytes in a wide variety of complex mixtures including fuels, industrial feedstocks, fragrances, and environmental extracts. Valve-based comprehensive 2-D GC (GC×GC) was also actively developed in the past decade. Valve-based modulation is a simple way to generate GC×GC separations without using cryogenic fluids. More than ten new valve-based modulators were introduced. Diaphragm valves fitted with sample loops are the most common low duty cycle modulators, whereas fluidic modulators that employ differential flow conditions are the most common high duty cycle modulators. Applications of valve-based GC×GC include analysis of hydrocarbon mixtures, essential oils, and environmental samples.
本文综述了流量控制二维气相色谱（2-D GC）的持续发展，特别强调了 2001 年至 2011 年期间发表的结果。中心切割二维气相色谱仍然用于从复杂混合物中分离选定的成分。现代气相色谱仪产生的可编程和高度精确的流量和温度使得更容易将分析物选择性地转移到第二根柱子上，并从第一根柱子上反冲洗不需要的成分。已经引入了几种用于执行中心切割二维气相色谱的新的Dean 切换接口，其中大多数注意力集中在将流量连接集成到单个单元中的设备上。中心切割二维气相色谱已用于分离各种复杂混合物中的分析物，包括燃料、工业原料、香料和环境提取物。基于阀的全二维气相色谱（GC×GC）在过去十年中也得到了积极的发展。基于阀的调制是一种无需使用低温液体即可产生 GC×GC 分离的简单方法。引入了十多种新的基于阀的调制器。带有样品环的隔膜阀是最常见的低占空比调制器，而采用差分流量条件的流体调制器是最常见的高占空比调制器。基于阀的 GC×GC 的应用包括烃混合物、精油和环境样品的分析。
