Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, British Columbia, Canada.
Bioresour Technol. 2011 Mar;102(6):4507-17. doi: 10.1016/j.biortech.2010.12.082. Epub 2010 Dec 30.
Douglas-fir was SO(2)-steam pretreated at different severities (190, 200, and 210°C) to assess the possible negative effect of the residual and isolated lignins on the enzymatic hydrolysis of the steam pretreated substrates. When various isolated lignins were added to the Avicel hydrolysis reactions, the decrease in glucose yields ranged from 15.2% to 29.0% after 72 h. It was apparent that the better hydrolysis yields obtained at higher pretreatment severities were more a result of the greater accessibly of the cellulose rather than any specific change in the non-productive binding of the lignin to the enzymes. FTIR and (13)C NMR characterization indicated that the lignin in the steam pretreated substrates became more condensed with increasing severity, suggesting that the cellulases were adsorbed to the lignin by hydrophobic interactions. Electrostatic interactions were also involved as the positively charged cellulase components were preferentially adsorbed to the lignins.
花旗松经不同程度的 SO(2)-蒸汽预处理（190、200 和 210°C），以评估残余和分离木质素对蒸汽预处理底物酶水解的可能负面影响。当各种分离木质素被添加到纤维素酶水解反应中时，72 小时后葡萄糖产率下降 15.2%至 29.0%。显然，在较高预处理程度下获得更好的水解产率更多是由于纤维素的可及性增加，而不是木质素与酶的非生产性结合发生任何特定变化的结果。傅里叶变换红外光谱（FTIR）和（13）C 核磁共振（NMR）表征表明，随着预处理程度的增加，蒸汽预处理底物中的木质素变得更加缩合，这表明纤维素酶通过疏水相互作用吸附到木质素上。静电相互作用也参与其中，因为带正电荷的纤维素酶成分优先吸附到木质素上。
