Forest Products Biotechnology/Bio-energy Research Group, Forest Sciences Centre, University of British Columbia, 2424-Main Mall, Vancouver, BC, Canada, V6T1Z4.
Bioresour Technol. 2012 Jan;103(1):201-8. doi: 10.1016/j.biortech.2011.09.091. Epub 2011 Oct 8.
The influence of cellulose accessibility and protein loading on the efficiency of enzymatic hydrolysis of steam pretreated Douglas-fir was assessed. It was apparent that the lignin component significantly influences the swelling/accessibility of cellulose as at low protein loadings (5FPU/g cellulose), only 16% of the cellulose present in the steam pretreated softwood was hydrolyzed while almost complete hydrolysis was achieved with the delignified substrate. When lignin (isolated from steam pretreated Douglas-fir) was added back in the same proportions it was originally found to the highly accessible and swollen, delignified steam pretreated softwood and to a cellulose control such as Avicel, the hydrolysis yields decreased by 9 and 46%, respectively. However, when higher enzyme loadings were employed, the greater availability of the enzyme could overcome the limitations imposed by both the lignin's restrictions on cellulose accessibility and direct binding of the enzymes, resulting in a near complete hydrolysis of the cellulose.
研究了纤维素可及性和蛋白质负载量对蒸汽预处理花旗松酶水解效率的影响。结果表明，木质素成分显著影响纤维素的溶胀/可及性，在低蛋白质负载量（5FPU/g 纤维素）下，蒸汽预处理软木中只有 16%的纤维素被水解，而脱木质素底物则几乎完全水解。当将木质素（从蒸汽预处理花旗松中分离得到）以其原始比例添加回高度可及和溶胀的脱木质素蒸汽预处理软木以及纤维素对照物（如 Avicel）中时，水解产率分别降低了 9%和 46%。然而，当使用更高的酶负载量时，酶的更大可用性可以克服木质素对纤维素可及性的限制以及酶的直接结合所带来的限制，从而导致纤维素几乎完全水解。
