Unité de Recherches sur les Herbivores, Institut National de la Recherche Agronomique, Theix, Saint Genès-Champanelle, France.
Comp Biochem Physiol Part D Genomics Proteomics. 2011 Jun;6(2):139-49. doi: 10.1016/j.cbd.2010.12.002. Epub 2010 Dec 22.
A major function of ruminant adipose tissue is to store lipids for use in productive functions. Body fat mobilization is required during periods of negative energy balance such as lactation or undernutrition. Until now, gene expression profiling of ruminant adipose tissue in response to nutritional restriction has not been performed. To gain a better understanding of the molecular mechanisms in adipose tissue in response to dietary factors, microarray analysis was used to compare the effects of two extreme nutritional conditions (control diet vs. 48-h feed deprivation) in the omental and perirenal adipose tissues of lactating goats (Capra hircus). We observed the altered expression of 456 and 199 genes in omental and perirenal adipose tissues, respectively. Similar biological processes were altered by feed deprivation in these two sites, although twice as many genes were differentially expressed in the omental than in the perirenal adipose tissue. Taken together, the transcriptional changes involved in lipid metabolism (decreased lipid synthesis and triglyceride storage capacity as well as increased fatty acid oxidation) were consistent with reduced energy deposition in goat adipose tissues in response to a 48-h fast. An inflammatory state of the adipose tissue was observed following the 48-h fast.
反刍动物脂肪组织的主要功能是储存脂质，以供生产功能使用。在泌乳或营养不良等负能量平衡期间，需要动员体脂肪。到目前为止，还没有对反刍动物脂肪组织对营养限制的基因表达谱进行研究。为了更好地了解脂肪组织对饮食因素的分子机制，我们使用微阵列分析比较了两种极端营养条件（对照饮食与 48 小时禁食）对泌乳山羊（Capra hircus）网膜和肾周脂肪组织的影响。我们观察到网膜和肾周脂肪组织中分别有 456 个和 199 个基因的表达发生改变。尽管在网膜脂肪组织中差异表达的基因数量是肾周脂肪组织的两倍，但禁食在这两个部位改变了相似的生物学过程。总的来说，与脂质代谢相关的转录变化（降低脂质合成和甘油三酯储存能力，增加脂肪酸氧化）与山羊脂肪组织对 48 小时禁食的能量沉积减少一致。在禁食 48 小时后，观察到脂肪组织处于炎症状态。
