INSERM UMRS, University of Nantes, Laboratory of Osteo-articular and Dental Tissue Engineering, School of Dental Surgery, France.
Acta Biomater. 2011 May;7(5):2119-30. doi: 10.1016/j.actbio.2011.01.025. Epub 2011 Jan 21.
Natural polysaccharides are attractive compounds with which to build scaffolds for bone and cartilage tissue engineering. Here we tested two non-standard ones, HE800 and GY785, for the two-dimensional (2-D) and three-dimensional (3-D) culture of osteoblasts (MC3T3-E1) and chondrocytes (C28/I2). These two glycosaminoglycan-like marine exopolysaccharides were incorporated into an injectable silylated hydroxypropylmethylcellulose-based hydrogel (Si-HPMC) that has already shown its suitability for bone and cartilage tissue engineering. Results showed that, similarly to hyaluronic acid (HA) (the control), HE800 and GY785 significantly improved the mechanical properties of the Si-HPMC hydrogel and induced the attachment of MC3T3-E1 and C28/I2 cells when these were cultured on top of the scaffolds. Si-HPMC hydrogel containing 0.67% HE800 exhibited the highest compressive modulus (11kPa) and allowed the best cell dispersion, especially of MC3T3-E1 cells. However, these cells did not survive when cultured in 3-D within hydrogels containing HE800, in contrast to C28/I2 cells. The latter proliferated in the microenvironment or concentrically depending on the nature of the hydrogel. Among all the constructs tested the Si-HPMC hydrogels containing 0.34% HE800 or 0.67% GY785 or 0.67% HA presented the most interesting features for cartilage tissue engineering applications, since they offered the highest compressive modulus (9.5-11kPa) while supporting the proliferation of chondrocytes.
天然多糖是构建骨和软骨组织工程支架的有吸引力的化合物。在这里，我们测试了两种非标准多糖 HE800 和 GY785，用于成骨细胞（MC3T3-E1）和软骨细胞（C28/I2）的二维（2-D）和三维（3-D）培养。这两种糖胺聚糖样海洋胞外多糖被掺入到一种可注射的硅烷化羟丙基甲基纤维素基水凝胶（Si-HPMC）中，该水凝胶已经显示出其在骨和软骨组织工程中的适用性。结果表明，与透明质酸（HA）（对照）类似，HE800 和 GY785 显著改善了 Si-HPMC 水凝胶的机械性能，并在支架顶部培养 MC3T3-E1 和 C28/I2 细胞时诱导其附着。含有 0.67%HE800 的 Si-HPMC 水凝胶表现出最高的压缩模量（11kPa），并允许最好的细胞分散，特别是 MC3T3-E1 细胞。然而，当在含有 HE800 的水凝胶中进行 3-D 培养时，这些细胞不能存活，而 C28/I2 细胞则可以。后者在微环境中或根据水凝胶的性质同心地增殖。在所有测试的构建体中，含有 0.34%HE800 或 0.67%GY785 或 0.67%HA 的 Si-HPMC 水凝胶表现出最有趣的软骨组织工程应用特征，因为它们提供了最高的压缩模量（9.5-11kPa），同时支持软骨细胞的增殖。
