Réthoré Gildas, Boyer Cécile, Kouadio Kouakou, Toure Amadou, Lesoeur Julie, Halgand Boris, Jordana Fabienne, Guicheux Jérôme, Weiss Pierre
Dental Faculty, Université de Nantes, UMR 1229, RMeS, Regenerative Medicine and Skeleton, INSERM, ONIRIS, F-44042 Nantes, France.
Institut National de la Santé et de la Recherche Médicale, Université de Nantes, UFR Odontologie, F-44042 Nantes, France.
Polymers (Basel). 2020 Nov 27;12(12):2823. doi: 10.3390/polym12122823.
Tissue engineering is a multidisciplinary field that relies on the development of customized biomaterial to support cell growth, differentiation and matrix production. Toward that goal, we designed the grafting of silane groups onto the chitosan backbone (Si-chito) for the preparation of in situ setting hydrogels in association with silanized hydroxypropyl methylcellulose (Si-HPMC). Once functionalized, the chitosan was characterized, and the presence of silane groups and its ability to gel were demonstrated by rheology that strongly suggests the presence of silane groups. Throughout physicochemical investigations, the Si-HPMC hydrogels containing Si-chito were found to be stiffer with an injection force unmodified. The presence of chitosan within the hydrogel has demonstrated a higher adhesion of the hydrogel onto the surface of tissues. The results of cell viability assays indicated that there was no cytotoxicity of Si-chito hydrogels in 2D and 3D culture of human SW1353 cells and human adipose stromal cells, respectively. Moreover, Si-chito allows the transplantation of human nasal chondrocytes in the subcutis of nude mice while maintaining their viability and extracellular matrix secretory activity. To conclude, Si-chito mixed with Si-HPMC is an injectable, self-setting and cytocompatible hydrogel able to support the in vitro and in vivo viability and activity of hASC.
组织工程是一个多学科领域，它依赖于定制生物材料的开发来支持细胞生长、分化和基质产生。为了实现这一目标，我们设计了将硅烷基团接枝到壳聚糖主链上（Si-chito），以便与硅烷化羟丙基甲基纤维素（Si-HPMC）联合制备原位固化水凝胶。功能化后，对壳聚糖进行了表征，流变学证明了硅烷基团的存在及其凝胶化能力，这强烈表明存在硅烷基团。在整个物理化学研究中，发现含有Si-chito的Si-HPMC水凝胶更硬，而注射力未改变。水凝胶中壳聚糖的存在证明了水凝胶在组织表面具有更高的粘附性。细胞活力测定结果表明，Si-chito水凝胶在人SW1353细胞和人脂肪基质细胞的二维和三维培养中分别没有细胞毒性。此外，Si-chito允许将人鼻软骨细胞移植到裸鼠皮下，同时保持其活力和细胞外基质分泌活性。总之，与Si-HPMC混合的Si-chito是一种可注射、自固化且具有细胞相容性的水凝胶，能够支持人脂肪干细胞在体外和体内的活力和活性。
