Lebellenger Lou, Verrez-Bagnis Véronique, Passerini Delphine, Delbarre-Ladrat Christine
Ifremer, Atlantique Center, Biotechnology and Marine Resources Unit, Marine Ecosystems and Marine Molecules for the Biotechnologies Laboratory, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes Cedex 3, France.
BMC Res Notes. 2018 Feb 6;11(1):102. doi: 10.1186/s13104-018-3214-z.
The eps locus in Vibrio diabolicus is involved in the production of the biotechnologically valuable HE800 EPS. In this study, the distribution and diversity of similar eps gene clusters across Vibrionaceae and its variability in relation to phylogenetic relationship were investigated. The aim was to provide a better knowledge of the eps gene cluster importance and to facilitate discovery of new EPS with potent interesting bioactivities.
Seventy percent of the 103 genome sequences examined display such an eps locus with a high level of synteny. However, genetic divergence was found inside some monophyletic clades or even between some strains of the same species. It includes gene insertions, truncations, and deletions. Comparative analysis also reveals some variations in glycosyltransferase and export systems genes. Phylogenetic analysis of the Vibrionaceae eps gene clusters within Vibrionaceae suggests a vertical transfer by speciation but also pinpoints rearrangement events independent of the speciation.
“恶魔弧菌”（Vibrio diabolicus）中的eps基因座参与了具有生物技术价值的HE800胞外多糖（EPS）的产生。在本研究中，对弧菌科中相似eps基因簇的分布和多样性及其与系统发育关系相关的变异性进行了研究。目的是更好地了解eps基因簇的重要性，并促进发现具有强大有趣生物活性的新EPS。
在所检测的103个基因组序列中，70%显示出这样一个具有高度同线性的eps基因座。然而，在一些单系分支内部甚至同一物种的一些菌株之间发现了遗传差异。这包括基因插入、截断和缺失。比较分析还揭示了糖基转移酶和输出系统基因的一些变异。弧菌科内弧菌科eps基因簇的系统发育分析表明通过物种形成进行垂直转移，但也指出了独立于物种形成的重排事件。
