Shelton Ryan L, Applegate Brian E
Department of Biomedical Engineering, Texas A&M University, 337 Zachry Engineering Center, TAMU 3120, College Station, TX 77843, USA.
Biomed Opt Express. 2010 Aug 23;1(2):676-686. doi: 10.1364/BOE.1.000676.
We have developed a novel, hybrid imaging modality, Transient Absorption Ultrasonic Microscopy (TAUM), which takes advantage of the optical nonlinearities afforded by transient absorption to achieve ultrahigh-resolution photoacoustic microscopy. The theoretical point spread function for TAUM is functionally equivalent to confocal and two-photon fluorescence microscopy, potentially enabling cellular/subcellular photoacoustic imaging. A prototype TAUM system was designed, built, and used to image a cross-section through several capillaries in the excised cheek pouch of a Syrian Hamster. The well-resolved capillaries in the TAUM image provided experimental evidence of the spatial resolution. These results suggest that TAUM has excellent potential for producing volumetric images with cellular/subcellular resolution in three dimensions deep inside living tissue.
我们开发了一种新型的混合成像模式，即瞬态吸收超声显微镜（TAUM），它利用瞬态吸收所提供的光学非线性来实现超高分辨率光声显微镜成像。TAUM的理论点扩散函数在功能上等同于共聚焦和双光子荧光显微镜，有可能实现细胞/亚细胞光声成像。设计、构建了一个TAUM原型系统，并用于对叙利亚仓鼠切除的颊囊中的几根毛细血管的横截面进行成像。TAUM图像中分辨率良好的毛细血管提供了空间分辨率的实验证据。这些结果表明，TAUM在生成活体组织内部深处三维的细胞/亚细胞分辨率的体积图像方面具有出色的潜力。
