Zhang Jinying, Shi Yifan, Zhang Yexiaotong, Liu Haoran, Li Shihao, Liu Linglu
Beijing Key Laboratory for Precision Optoelectronic Measurement Instrument and Technology, School of Optics and Photonics, Beijing Institute of Technology, Beijing 100081, China.
Yangtze Delta Region Academy of Beijing Institute of Technology, Jiaxing 314001, China.
Micromachines (Basel). 2024 Nov 30;15(12):1463. doi: 10.3390/mi15121463.
Photoacoustic imaging has emerged as a promising modality for medical imaging since its introduction. Photoacoustic microscopy (PAM), which is based on the photoacoustic effect, combines the advantages of both optical and acoustic imaging modalities. PAM facilitates high-sensitivity, high-resolution, non-contact, and non-invasive imaging by employing optical absorption as its primary contrast mechanism. The ability of PAM to specifically image parameters such as blood oxygenation and melanin content makes it a valuable addition to the suite of modern biomedical imaging techniques. This review aims to provide a comprehensive overview of the diverse technical approaches and methods employed by researchers to enhance the resolution of photoacoustic microscopy. Firstly, the fundamental principles of the photoacoustic effect and photoacoustic imaging will be presented. Subsequently, resolution enhancement methods for both acoustic-resolution photoacoustic microscopy (AR-PAM) and optical-resolution photoacoustic microscopy (OR-PAM) will be discussed independently. Finally, the aforementioned resolution enhancement methods for photoacoustic microscopy will be critically evaluated, and the current challenges and future prospects of this technology will be summarized.
自引入以来，光声成像已成为一种很有前景的医学成像方式。基于光声效应的光声显微镜（PAM）结合了光学和声学成像方式的优点。PAM通过采用光吸收作为其主要对比机制，实现了高灵敏度、高分辨率、非接触和非侵入性成像。PAM能够特异性地对诸如血氧饱和度和黑色素含量等参数进行成像，这使其成为现代生物医学成像技术中的一项重要补充。本综述旨在全面概述研究人员为提高光声显微镜分辨率所采用的各种技术方法。首先，将介绍光声效应和光声成像的基本原理。随后，将分别讨论声学分辨率光声显微镜（AR-PAM）和光学分辨率光声显微镜（OR-PAM）的分辨率增强方法。最后，将对上述光声显微镜分辨率增强方法进行批判性评估，并总结该技术当前面临的挑战和未来前景。
